LOCUS       40924_34485        7436 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cosmid vector pPIBTcosCAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 7436)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7436)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..546
                     /label=contains Ulva mutabilis RbcS promoter and 5' UTR
                     /note="contains Ulva mutabilis RbcS promoter and 5' UTR"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     3'UTR           1419..1807
                     /label=Ulva mutabilis RbcS 3' UTR
                     /note="Ulva mutabilis RbcS 3' UTR"
     misc_feature    1816..1986
                     /label=cloning vector pUC18, with minor modifications
                     /note="cloning vector pUC18, with minor modifications"
     CDS             complement(4031..4687)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4688..4790)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     primer_bind     5301..5317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5321..5377)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5387..5403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5411..5427)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5435..5465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5480..5501)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5789..6377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6551..7408)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt
     1441 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     1501 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     1561 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     1621 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     1681 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     1741 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     1801 aaaccggggg aattattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1861 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1921 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1981 cacgaggcca attcggatct gcctgctggc ctgccgcagt tcttcaacct cccggcgcag
     2041 cttttcgttc tcaatttcag catccctttc ggcataccat tttatgacgg cggcagagtc
     2101 ataaagcacc tcattaccct tgccaccgcc tcgcagaacg ggcattccct gttcctgcca
     2161 gttctgaatg gtacggatac tcgcaccgaa aatgtcagcc actgcttttt gttgacttcc
     2221 attgttcatt ccacggacaa aaacagagaa aggaaacgac agaggccaaa aagctcgctt
     2281 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg
     2341 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga
     2401 ggtcgccgcc ccgtaacaag gcggatcgcc ggaaaggacc cgcaaatgat aataattatc
     2461 aattgcatac tatcgacggc actgctgcca gataacacca ccggggaaac attccatcat
     2521 gatggccgtg cggacatagg aagccagttc atccatcgct ttcttgtctg ctgccatttg
     2581 ctttgtgaca tccagcgccg cacattcagc agcgtttttc agcgcgtttt cgatcaacgt
     2641 ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga cggttacaat
     2701 ttccggatct gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt
     2761 ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac
     2821 ctcattaccc ttgccaccgc ctcgcaaaac gggcattccc tgttcctgcc agttctgaat
     2881 ggtacggata ctcgcaccga aaatgtcagc cagctaggga taacagggta atctgctttt
     2941 tgttgacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa
     3001 aaagctcgct ttcagcacct gtcgtttcct ttcttttcag agggtatttt aaataaaaac
     3061 attaagttat gacgaagaag aacggaaacg ccttaaaccg gaaaattttc ataaatagcg
     3121 aaaacccgcg aggtcgccgc cccgtaacaa ggcggatcgc cggaaaggac ccgcaaatga
     3181 taataattat caattgcata ctatcgacgg cactgctgcc agataacacc accggggaaa
     3241 cattccatca tgatggccgt gcggacatag gaagccagtt catccatcgc tttcttgtct
     3301 gctgccattt gctttgtgac atccagcgcc gcacattcag cagcgttttt cagcgcgttt
     3361 tcgatcaacg tttcaatgtt ggtatcaaca ccaggtttaa ctttgaactt atcggcactg
     3421 acggttaccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt
     3481 ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct
     3541 tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg
     3601 ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc
     3661 catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt
     3721 ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg
     3781 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     3841 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgacataag cggctattta
     3901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     3961 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
     4021 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     4081 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     4141 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     4201 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     4261 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     4321 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     4381 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     4441 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     4501 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     4561 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     4621 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     4681 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     4741 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     4801 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     4861 ctgcgaagtg atcttccgtc acacgatgat aagctgtcaa acatgagaat tgccctttcg
     4921 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4981 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     5041 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     5101 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     5161 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5221 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     5281 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt
     5341 cgactctaga ggatccccgg gtaccgagct cgaattcgta atcatggtca tagctgtttc
     5401 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5461 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5581 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5641 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5701 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5761 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5821 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5881 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5941 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6001 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6061 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6121 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6181 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6241 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6301 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6361 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6421 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6481 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6541 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6601 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6661 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6721 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6781 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6841 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6901 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6961 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7021 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7081 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7141 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7201 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7261 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7321 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7381 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaataattc ccatgg
//
