Basic Vector Information
- Vector Name:
- pPIBT3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4511 bp
- Type:
- Transformation vector
- Replication origin:
- ori
- Source/Author:
- Oertel W, Weissgerber A.
pPIBT3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIBT3 vector Sequence
LOCUS 40924_34475 4511 bp DNA circular SYN 18-DEC-2018
DEFINITION Transformation vector pPIBT3, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4511)
AUTHORS Oertel W, Weissgerber A.
TITLE Transformation of Ulva mutabilis Foyn with plasmids integrating into
the chromosome
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4511)
AUTHORS Oertel W, Weissgerber A.
TITLE Direct Submission
JOURNAL Submitted (25-SEP-2007) Biochemistry, Genetics and Microbiology,
Division of Genetics, University of Regensburg, Universitaetsstr.
31, Regensburg, Bavaria 93053, Germany
REFERENCE 3 (bases 1 to 4511)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4511)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(25-SEP-2007) Biochemistry, Genetics and Microbiology, Division of
Genetics, University of Regensburg, Universitaetsstr. 31,
Regensburg, Bavaria 93053, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4511
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 1..546
/label=Ulva mutabilis RbcS promoter
/note="Ulva mutabilis RbcS promoter"
/regulatory_class="promoter"
intron 555..1051
/gene="ble"
/note="Ulva mutabilis RbcS intron 1; enhancer"
CDS 1050..1415
/label=BleoR
/note="antibiotic-binding protein"
regulatory 1419..1807
/label=Ulva mutabilis RbcS terminator
/note="Ulva mutabilis RbcS terminator"
/regulatory_class="terminator"
primer_bind 2376..2392
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(2396..2452)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(2462..2478)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2486..2502)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2510..2540)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2555..2576)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2864..3452)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3626..4483)
/label=AmpR
/note="beta-lactamase"
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