LOCUS       40924_34475        4511 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPIBT3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 4511)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4511)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva mutabilis RbcS promoter
                     /note="Ulva mutabilis RbcS promoter"
                     /regulatory_class="promoter"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      1419..1807
                     /label=Ulva mutabilis RbcS terminator
                     /note="Ulva mutabilis RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     2376..2392
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2396..2452)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2462..2478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2486..2502)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2510..2540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2555..2576)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2864..3452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3626..4483)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt
     1441 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     1501 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     1561 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     1621 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     1681 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     1741 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     1801 aaaccggggg aattattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1861 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1921 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1981 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     2041 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     2101 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     2161 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2221 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2281 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2341 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2401 tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tcgtaatcat
     2461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     2521 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     2581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     2641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     2701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3301 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     3601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     3961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     4321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     4381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     4441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     4501 aattcccatg g
//
