Basic Vector Information
- Vector Name:
- pPIBGT8
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5173 bp
- Type:
- Transformation vector
- Replication origin:
- ori
- Source/Author:
- Oertel W, Weissgerber A.
pPIBGT8 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPIBGT8 vector Sequence
LOCUS 40924_34470 5173 bp DNA circular SYN 18-DEC-2018
DEFINITION Transformation vector pPIBGT8, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5173)
AUTHORS Oertel W, Weissgerber A.
TITLE Transformation of Ulva mutabilis Foyn with plasmids integrating into
the chromosome
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5173)
AUTHORS Oertel W, Weissgerber A.
TITLE Direct Submission
JOURNAL Submitted (10-OCT-2007) Biochemistry, Genetics and Microbiology,
Division of Genetics, University of Regensburg, Universitaetsstr.
31, Regensburg, Bavaria 93053, Germany
REFERENCE 3 (bases 1 to 5173)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5173)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-OCT-2007) Biochemistry, Genetics and Microbiology, Division of
Genetics, University of Regensburg, Universitaetsstr. 31,
Regensburg, Bavaria 93053, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5173
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 1..546
/label=Ulva RbcS promoter
/note="Ulva RbcS promoter"
/regulatory_class="promoter"
intron 555..1051
/gene="ble/GFP fusion"
/number=1
/note="derived from Ulva RbcS; enhancer"
CDS 1413..2126
/label=EGFP
/note="enhanced GFP"
regulatory 2139..2527
/label=Ulva RbcS terminator
/note="Ulva RbcS terminator"
/regulatory_class="terminator"
primer_bind complement(2536..2552)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3026..3130
/label=AmpR promoter
CDS 3131..3988
/label=AmpR
/note="beta-lactamase"
rep_origin 4162..4750
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5038..5059
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5074..5104
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5112..5128
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5136..5152
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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