LOCUS       40924_34470        5173 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPIBGT8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 5173)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5173)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva RbcS promoter
                     /note="Ulva RbcS promoter"
                     /regulatory_class="promoter"
     intron          555..1051
                     /gene="ble/GFP fusion"
                     /number=1
                     /note="derived from Ulva RbcS; enhancer"
     CDS             1413..2126
                     /label=EGFP
                     /note="enhanced GFP"
     regulatory      2139..2527
                     /label=Ulva RbcS terminator
                     /note="Ulva RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2536..2552)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3026..3130
                     /label=AmpR promoter
     CDS             3131..3988
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4162..4750
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5038..5059
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5074..5104
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5112..5128
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5136..5152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggtacccaa gggcgaggag ctgttcaccg
     1441 gtgtggtccc catcctggtg gagctggacg gcgacgtgaa cggccacaag ttctccgtct
     1501 ccggcgaggg tgagggtgac gccacctacg gcaagctgac cctgaagttc atctgcacca
     1561 ccggcaagct gcccgtgccc tggcccaccc tggtcaccac cctgacctac ggtgtgcagt
     1621 gcttctcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1681 agggctacgt gcaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1741 ccgaggtcaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1801 tcaaggagga cggcaacatc ctgggccaca agctggagta caactacaac tcccacaacg
     1861 tgtacatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1921 acatcgagga cggctccgtg cagctggccg accactacca gcagaacacc cccatcggcg
     1981 atggccccgt gctgctgccc gacaaccact acctgtccat ccagtccgcc ctgtccaagg
     2041 accccaacga gaagcgcgac cacatggtcc tgctggagtt cgtcaccgct gccggcatca
     2101 cccacggcat ggacgagctg tacaagtaaa ggatccccgc agtcaggcag agtcgttgtt
     2161 gggtgtatgg ttgcacaaaa ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     2221 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     2281 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     2341 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     2401 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     2461 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     2521 aaaccggagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2581 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2641 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     2701 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     2761 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     2821 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2881 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     2941 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     3001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     4321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5161 cgaattccca tgg
//
