Basic Vector Information
- Vector Name:
- pPGKneo-I
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5568 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Graubert TA, Lu ZH, Ley TJ.
- Promoter:
- SP6
pPGKneo-I vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pPGKneo-I vector Sequence
LOCUS 40924_34386 5568 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPGKneo-I, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5568)
AUTHORS Graubert TA, Lu ZH, Ley TJ.
TITLE Direct Submission
JOURNAL Submitted (11-JAN-2001) Division of Oncology, Section of Stem Cell
Biology, Washington University School of Medicine, 660 South Euclid
Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE 2 (bases 1 to 5568)
AUTHORS Graubert TA, Lu ZH, Ley TJ.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2002) Division of Oncology, Section of Stem Cell
Biology, Washington University School of Medicine, 660 South Euclid
Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE 3 (bases 1 to 5568)
AUTHORS Graubert TA, Lu ZH, Ley TJ.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2002) Division of Oncology, Section of Stem Cell
Biology, Washington University School of Medicine, 660 South Euclid
Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE 4 (bases 1 to 5568)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 5568)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(11-JAN-2001) Division of Oncology, Section of Stem Cell Biology,
Washington University School of Medicine, 660 South Euclid Avenue,
Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-MAR-2002) Division of Oncology, Section of Stem Cell Biology,
Washington University School of Medicine, 660 South Euclid Avenue,
Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(25-JUL-2002) Division of Oncology, Section of Stem Cell Biology,
Washington University School of Medicine, 660 South Euclid Avenue,
Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On Jul 25, 2002 this sequence version replaced AF335419.2.
FEATURES Location/Qualifiers
source 1..5568
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 239..257
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
misc_feature 338..371
/label=lox-P site
/note="lox-P site"
protein_bind complement(338..371)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 372..867
/label=PGK
/note="PGK"
/regulatory_class="promoter"
CDS 876..1676
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase from Tn5"
polyA_signal 1717..1828
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature 1916..1949
/label=lox-P site
/note="lox-P site"
protein_bind complement(1916..1949)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter complement(2025..2043)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2050..2066)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2208..2588
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS 2956..3747
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
CDS 3768..4625
/label=AmpR
/note="beta-lactamase"
rep_origin 4799..5387
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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