pPGKneo-I vector (V004122)

LOCUS       40924_34386        5568 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPGKneo-I, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2001) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2002) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   3  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2002) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   4  (bases 1 to 5568)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-JAN-2001) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2002) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2002) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jul 25, 2002 this sequence version replaced AF335419.2.
FEATURES             Location/Qualifiers
     source          1..5568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    338..371
                     /label=lox-P site
                     /note="lox-P site"
     protein_bind    complement(338..371)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      372..867
                     /label=PGK
                     /note="PGK"
                     /regulatory_class="promoter"
     CDS             876..1676
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1717..1828
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1916..1949
                     /label=lox-P site
                     /note="lox-P site"
     protein_bind    complement(1916..1949)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(2025..2043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2050..2066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2208..2588
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2956..3747
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3768..4625
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4799..5387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"