LOCUS       40924_34386        5568 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPGKneo-I, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2001) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2002) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   3  (bases 1 to 5568)
  AUTHORS   Graubert TA, Lu ZH, Ley TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2002) Division of Oncology, Section of Stem Cell 
            Biology, Washington University School of Medicine, 660 South Euclid 
            Avenue, Campus Box 8007, St. Louis, MO 63110-1093, USA
REFERENCE   4  (bases 1 to 5568)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-JAN-2001) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2002) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2002) Division of Oncology, Section of Stem Cell Biology, 
            Washington University School of Medicine, 660 South Euclid Avenue, 
            Campus Box 8007, St. Louis, MO 63110-1093, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Jul 25, 2002 this sequence version replaced AF335419.2.
FEATURES             Location/Qualifiers
     source          1..5568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    338..371
                     /label=lox-P site
                     /note="lox-P site"
     protein_bind    complement(338..371)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      372..867
                     /label=PGK
                     /note="PGK"
                     /regulatory_class="promoter"
     CDS             876..1676
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1717..1828
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1916..1949
                     /label=lox-P site
                     /note="lox-P site"
     protein_bind    complement(1916..1949)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(2025..2043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2050..2066)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2208..2588
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             2956..3747
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3768..4625
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4799..5387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttcaaca acttcgtata atgtatgcta
      361 tacgaagtta tcagtacttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      421 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      481 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      541 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      601 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
      661 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
      721 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
      781 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
      841 ttcctcatct ccgggccttt cgacctgcag ccaatatggg atcggccatt gaacaagatg
      901 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
      961 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     1021 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     1081 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     1141 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     1201 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     1261 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     1321 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     1381 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     1441 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     1501 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     1561 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     1621 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     1681 gggatcaatt ctctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     1741 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     1801 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     1861 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcaacaac
     1921 ttcgtataat gtatgctata cgaagttatc agtacaagcc gaattctgca gatatccatc
     1981 acactggcgg ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta
     2041 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2101 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2161 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     2221 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2281 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2341 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagag ctttacggca
     2401 cctcgaccgc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     2461 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2521 aactggaaca acactcaacc ctatcgcggt ctattctttt gatttataag ggattttgcc
     2581 gatttcggcc tattggttaa aaaatgagct gatttaacaa attcagggcg caagggctgc
     2641 taaaggaacc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     2701 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct
     2761 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg
     2821 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg
     2881 gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca agagacagga
     2941 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3001 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3061 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3121 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3181 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3241 gaagtgccgg ggcaggatct cctgtcatct cgccttgctc ctgccgagaa agtatccatc
     3301 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3361 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3421 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3481 gcgcgcatgc ccgacggcga ggatctcgtc gtgatccatg gcgatgcctg cttgccgaat
     3541 atcatggtgg aaaatggccg cttttctgga ttcaacgact gtggccggct gggtgtggcg
     3601 gaccgctatc aggacatagc gttggatacc cgtgatattg ctgaagagct tggcggcgaa
     3661 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3721 ttctatcgcc ttcttgacga gttcttctga attgaaaaag gaagagtatg agtattcaac
     3781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3841 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3961 caatgatgag cacttttaaa gttctgctat gtcatacact attatcccgt attgacgccg
     4021 ggcaagagca actcggtcgc cgggcgcggt attctcagaa tgacttggtt gagtactcac
     4081 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4141 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4201 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4261 cggagctgaa tgaagccata ccaaacgacg agagtgacac cacgatgcct gtagcaatgc
     4321 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4381 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4441 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4501 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4561 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4621 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4921 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5161 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     5221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5461 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5521 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
