pPANK vector (V004178)

LOCUS       40924_34086        3474 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPANK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Binz HK, Stumpp MT, Forrer P, Amstutz P, Pluckthun A.
  TITLE     Designing repeat proteins: well-expressed, soluble and stable 
            proteins from combinatorial libraries of consensus ankyrin repeat 
            proteins
  JOURNAL   J. Mol. Biol. 332 (2), 489-503 (2003)
  PUBMED    12948497
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Binz KH, Stumpp MT, Forrer P, Amstutz P, Zahnd C, Pluckthun A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   3  (bases 1 to 3474)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "2003"; volume: "332"; issue: "2"; pages: "489-503"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    145..198
                     /note="mcs; multiple cloning site"
     terminator      208..302
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             346..1002
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1070..1156
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1324..1464
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1650..2238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2412..3269)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3270..3374)
                     /label=AmpR promoter