LOCUS       40924_34086        3474 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPANK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Binz HK, Stumpp MT, Forrer P, Amstutz P, Pluckthun A.
  TITLE     Designing repeat proteins: well-expressed, soluble and stable 
            proteins from combinatorial libraries of consensus ankyrin repeat 
            proteins
  JOURNAL   J. Mol. Biol. 332 (2), 489-503 (2003)
  PUBMED    12948497
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Binz KH, Stumpp MT, Forrer P, Amstutz P, Zahnd C, Pluckthun A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   3  (bases 1 to 3474)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "2003"; volume: "332"; issue: "2"; pages: "489-503"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    145..198
                     /note="mcs; multiple cloning site"
     terminator      208..302
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             346..1002
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1070..1156
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1324..1464
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1650..2238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2412..3269)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3270..3374)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct
      181 gcagccaagc ttaattagct gagcttggac tcctgttgat agatccagta atgacctcag
      241 aactccatct ggatttgttc agaacgctcg gttgccgccg ggcgtttttt attggtgaga
      301 atccaagcta gcttggcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      541 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
      601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      781 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      841 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      901 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
      961 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1021 tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc aaataaaacg
     1081 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1141 cctgagtagg acaaatccgc cgctctagag ggaaacctag ggctgcctcg cgcgtttcgg
     1201 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1261 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     1321 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     1381 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1441 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1501 tcggtctgtc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1561 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2461 tcatccatag ctgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2521 tctggcccca gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca
     2581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3421 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgact acac
//
