pPAM vector (V004179)

MSDS

Basic Vector Information

Vector Name:: pPAM

Antibiotic Resistance:: Ampicillin

Length:: 3098 bp

Type:: Binary vector

Replication origin:: oriV

Source/Author:: Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, Kreuzaler F, Peterhansel C.

pPAM vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pPAM vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_34061        3098 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPAM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, 
            Kreuzaler F, Peterhansel C.
  TITLE     An engineered phosphoenolpyruvate carboxylase redirects carbon and 
            nitrogen flow in transgenic potato plants
  JOURNAL   Plant J. 32 (1), 25-39 (2002)
  PUBMED    12366798
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Rademacher TR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2001) Institut Fuer Biologie I, RWTH Aachen, 
            Worringer Weg 1, Aachen 52074, Germany
REFERENCE   3  (bases 1 to 3098)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2002"; volume: "32"; issue: "1"; pages: "25-39"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2001) Institut Fuer Biologie I, RWTH Aachen, Worringer Weg 
            1, Aachen 52074, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    89..113
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      225..934
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1007..1098
                     /label=AmpR promoter
     CDS             1099..1956
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2130..2718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3026..3050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"

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