LOCUS       40924_34061        3098 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPAM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Rademacher T, Hausler RE, Hirsch HJ, Zhang L, Lipka V, Weier D, 
            Kreuzaler F, Peterhansel C.
  TITLE     An engineered phosphoenolpyruvate carboxylase redirects carbon and 
            nitrogen flow in transgenic potato plants
  JOURNAL   Plant J. 32 (1), 25-39 (2002)
  PUBMED    12366798
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Rademacher TR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2001) Institut Fuer Biologie I, RWTH Aachen, 
            Worringer Weg 1, Aachen 52074, Germany
REFERENCE   3  (bases 1 to 3098)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3098)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2002"; volume: "32"; issue: "1"; pages: "25-39"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2001) Institut Fuer Biologie I, RWTH Aachen, Worringer Weg 
            1, Aachen 52074, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    89..113
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      225..934
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1007..1098
                     /label=AmpR promoter
     CDS             1099..1956
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2130..2718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3026..3050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 atcgaattcg gcgcgcccgg gcggccgcca ccgcggtgga gggggatcag attgtcgttt
       61 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
      121 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
      181 gtccatttgt atgtgtacat caccgacgag caaggcaaga ccgagcgcct ttccgacgct
      241 caccgggctg gttgccctcg ccgctgggct ggcggccgtc tatggccctg caaacgcgcc
      301 agaaacgccg tcgaagccgt gtgcgagaca ccggccgccg gcgttgtgga tacctcgcgg
      361 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
      421 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
      481 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
      541 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
      601 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
      661 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
      721 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
      781 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
      841 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
      901 tcggccgcga acggcctcac cccaaaaatg gcagcgctgg cagtccttgc cattgccggg
      961 atcggggcag taacgggatg ggcgatcagc ccgaaagcta cccctatttg tttatttttc
     1021 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1081 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1141 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1201 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1261 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1321 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1381 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1441 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1501 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1561 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1621 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1681 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1741 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1801 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     1861 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1921 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1981 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2761 tggccttttg ctcacatgga ctctagagga tcacaggcag caacgctctg tcatcgttac
     2821 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     2881 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     2941 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     3001 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     3061 ttagacaact taataacaca ttgcggacgt ttttaatg
//
