pFS98-SV40-FPR1 vector (V006183)

LOCUS       V006183                 4672 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006183
VERSION     V006183
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4672)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4672)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..1054
                     /gene="FPR1"
                     /label="fMet-Leu-Phe receptor"
                     /note="fMet-Leu-Phe receptor from Homo sapiens. Accession#:
                     P21462"
     polyA_signal    complement(1103..1224)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2651..3508)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3509..3613)
                     /label="AmpR promoter"
     rep_origin      3640..4095
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4226..4274
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4288..4379
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        4434..4630
                     /label="SV40 promoter"
                     /note="SV40 early promoter"