LOCUS       V006183                 4672 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006183
VERSION     V006183
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4672)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4672)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..1054
                     /gene="FPR1"
                     /label="fMet-Leu-Phe receptor"
                     /note="fMet-Leu-Phe receptor from Homo sapiens. Accession#:
                     P21462"
     polyA_signal    complement(1103..1224)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2651..3508)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3509..3613)
                     /label="AmpR promoter"
     rep_origin      3640..4095
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4226..4274
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4288..4379
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        4434..4630
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
ORIGIN
        1 caagatggag acaaattcct ctctccccac gaacatctct ggagggacac ctgctgtatc
       61 tgctggctat ctcttcctgg atatcatcac ttatctggta tttgcagtca cctttgtcct
      121 cggggtcctg ggcaacgggc ttgtgatctg ggtggctgga ttccggatga cacacacagt
      181 caccaccatc agttacctga acctggccgt ggctgacttc tgtttcacct ccactttgcc
      241 attcttcatg gtcaggaagg ccatgggagg acattggcct ttcggctggt tcctgtgcaa
      301 attcgtcttt accatagtgg acatcaactt gttcggaagt gtcttcctga tcgccctcat
      361 tgctctggac cgctgtgttt gcgtcctgca tccagtctgg acccagaacc accgcaccgt
      421 gagcctggcc aagaaggtga tcattgggcc ctgggtgatg gctctgctcc tcacattgcc
      481 agttatcatt cgtgtgacta cagtacctgg taaaacgggg acagtagcct gcacttttaa
      541 cttttcgccc tggaccaacg accctaaaga gaggataaat gtggccgttg ccatgttgac
      601 ggtgagaggc atcatccggt tcatcattgg cttcagcgca cccatgtcca tcgttgctgt
      661 cagttatggg cttattgcca ccaagatcca caagcaaggc ttgattaagt ccagtcgtcc
      721 cttacgggtc ctctcctttg tcgcagcagc cttttttctc tgctggtccc catatcaggt
      781 ggtggccctt atagccacag tcagaatccg tgagttattg caaggcatgt acaaagaaat
      841 tggtattgca gtggatgtga caagtgccct ggccttcttc aacagctgcc tcaaccccat
      901 gctctatgtc ttcatgggcc aggacttccg ggagaggctg atccacgccc ttcccgccag
      961 tctggagagg gccctgaccg aggactcaac ccaaactagt gacacagcta ccaattctac
     1021 tttaccttct gcagaggtgg agttacaggc aaagtgactc gagtctagag tcggggcggc
     1081 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     1141 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1201 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1261 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     1321 gataaggatc tgaacgatgg agcggagaat gggcggaact gggcggagtt aggggcggga
     1381 tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     1441 ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     1501 tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     1561 cattccacag cggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     1621 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     1681 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     3661 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3721 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3781 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3841 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3901 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3961 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4021 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4081 taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4141 tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg
     4201 tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     4261 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
     4321 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta
     4381 tcgataggta ccgagctctt acgcgtgcta gcccgggctc gagatctgcg atctgcatct
     4441 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4501 cagttccgcc cattctccgc cccatcgctg actaattttt tttatttatg cagaggccga
     4561 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4621 cttttgcaaa aagcttcgaa tcgcgaattc tagggagacc caagctggct ag
//
