pFRT vector (V006195)

LOCUS       40924_20506        8006 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pFRT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     A new convenient Drosophila transformation vector bearing tandem 
            functional FRT sites - pFRT
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2003) Lab. Molecular Cytogenetics, The Institute 
            of Cytology and Genetics, SD RAS, Lavrentjeva 10, Novosibirsk 
            630090, Russia
REFERENCE   3  (bases 1 to 8006)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2003) Lab. Molecular Cytogenetics, The Institute of Cytology
            and Genetics, SD RAS, Lavrentjeva 10, Novosibirsk 630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..586
                     /label=P element 5' end
     gene            complement(597..4733)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4785..4832
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    4843..4892
                     /note="MCS; multiple cloning site"
     misc_binding    4893..4959
                     /label=Flp recombinase binding site
                     /bound_moiety="Flp recombinase"
                     /note="FRT; Flp recombinase target"
     protein_bind    4902..4949
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    5014..5246
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    5247..5748
                     /label=white linker sequence
                     /note="white linker sequence"
     promoter        6048..6152
                     /label=AmpR promoter
     CDS             6153..7010
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7184..7772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7779..8006
                     /label=white linker sequence
                     /note="white linker sequence"