LOCUS       40924_20506        8006 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pFRT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     A new convenient Drosophila transformation vector bearing tandem 
            functional FRT sites - pFRT
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Gortchakov AA, Schwartz YB, Demakov SA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2003) Lab. Molecular Cytogenetics, The Institute 
            of Cytology and Genetics, SD RAS, Lavrentjeva 10, Novosibirsk 
            630090, Russia
REFERENCE   3  (bases 1 to 8006)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2003) Lab. Molecular Cytogenetics, The Institute of Cytology
            and Genetics, SD RAS, Lavrentjeva 10, Novosibirsk 630090, Russia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..586
                     /label=P element 5' end
     gene            complement(597..4733)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4785..4832
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    4843..4892
                     /note="MCS; multiple cloning site"
     misc_binding    4893..4959
                     /label=Flp recombinase binding site
                     /bound_moiety="Flp recombinase"
                     /note="FRT; Flp recombinase target"
     protein_bind    4902..4949
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    5014..5246
                     /label=P element 3' end
                     /note="P element 3' end"
     misc_feature    5247..5748
                     /label=white linker sequence
                     /note="white linker sequence"
     promoter        6048..6152
                     /label=AmpR promoter
     CDS             6153..7010
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7184..7772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7779..8006
                     /label=white linker sequence
                     /note="white linker sequence"
ORIGIN
        1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt
       61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg
      121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag
      181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg
      241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg
      301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg
      361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata
      421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca
      481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa
      541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttaattctag
      601 ttccagtgaa atccaagcat tttctaaatt aaatgtattc ttattattat agttgttatt
      661 tttgatatat ataaacaaca ctattatgcc caccattttt ttgagatgca tctacacaag
      721 gaacaaacac tggatgtcac tttcagttca aattgtaacg ctaatcactc cgaacaggtc
      781 acaaaaaatt accttaaaaa gtcataatat taaattagaa taaatatagc tgtgagggaa
      841 atatatacaa atatattgga gcaaataaat tgtacataca aatatttatt actaatttct
      901 attgagacga aatgaaccac tcggaaccat ttgagcgaac cgaatcgcgc ggaactaacg
      961 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     1021 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     1081 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     1141 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     1201 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     1261 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     1321 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     1381 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     1441 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     1501 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     1561 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     1621 atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag agcaggaatg
     1681 gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg gcgcaggata
     1741 ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc aggcagttga
     1801 agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc gtgaagacca
     1861 gtggcactgt gagaaaaagc ggtaattcgg caatcgtttt gcccagaaag tatgtgtcac
     1921 agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag gtgaacacct
     1981 aaatcgaatc gattcattag aaagttagta aattattaat atgcaaatgt attctaaaca
     2041 agacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag gaagaggaag
     2101 atggctccgt tgatattcat cacgcccact tgcgtgagtt gttggcccaa aaagatgagg
     2161 ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat taatattcat
     2221 atcttcaaca tgttcgcgag ttaaatgaaa tttatttatt ttctgcaaaa ctataaacta
     2281 tacatctcat tgaaaaaaac taagaagggt gtggaatcag gcaattctaa ctaaaatcta
     2341 gcgaatttgt ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac
     2401 cgttgtctga ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga
     2461 tcgccacagg accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc
     2521 cggctgctcc agtggcttct ccaaattttt ggtggccaac aactgctcca tatcccgggc
     2581 tactttgcta atagcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc
     2641 ccgtccgggc acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg
     2701 acactgggca cccacgctgg ataggagttg agatgtaatg taatgctaga tacccttaat
     2761 aaacacatcg aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag
     2821 ctaccctgcc ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaagacg
     2881 gctgatgaat ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga
     2941 cgacgctgtg ggcggtaaat gagtccagtc cggaggtggg ctcatcgcag atcagaagcg
     3001 gcggatcggt tagtgcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac
     3061 ctttcaccct gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct
     3121 ggatcacctg atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca
     3181 ccatggcctg gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct
     3241 gctggacata ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca
     3301 gtcgcatccc ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca
     3361 gcagggtcgt ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg
     3421 ccacgccgca aactgagttt caaattggta attggaccct ttattaagat ttcacacaga
     3481 tcagccgact gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat
     3541 gtgtcgctcg ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc
     3601 cggctgattg accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc
     3661 tagttggccg ctccctgaac cggagtcctc cggcggactg ggtggcagga gcgtgccgta
     3721 gtttttggcc tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc
     3781 ctgcaatgat aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac
     3841 aaaacgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat
     3901 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatgtagtcg aaaatgaatg
     3961 cctttagatg tcttgatcat gatatgatct taaaaattgt cttatatagc gagcacagct
     4021 accagaataa tctgtttcgt gtcactattt gtttgtgcga ttgcggtttg ggatttttgt
     4081 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa
     4141 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt
     4201 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc
     4261 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa
     4321 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa
     4381 ccgcttggag cttcgtcacg aaaccgctga caaagtgcaa ctgaaggcgg acattgacgc
     4441 taggtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg
     4501 atatttctgt ggacagagaa ggaggcaaac agcgctgact ttgagtggaa tgtcattttg
     4561 agtgagaggt aatcgaaaga acctggtact tcaaataccc ttggatcgaa gtaaatttaa
     4621 aactgatcag ataagttcaa tgatgtccag tgcagtaaaa aataaaaaaa aaatatgttt
     4681 ttttaaatct acattctcca aaaaagggtt ttattaactt acatacatac tagaattagg
     4741 ccttctagac cgcggccgca gatccccggg tacccgggga tcttgaagtt cctattccga
     4801 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagcta gcttgcatgc
     4861 tcctctagct agactcgagg aattcggatc cgggggatct tgaagttcct attccgaagt
     4921 tcctattctc tagaaagtat aggaacttca gagcgctttt gaagctgggt acccggggat
     4981 cgatcctcta gagtcgacct gcagcccaag ctttgcgtac tcgcaaatta ttaaaaataa
     5041 aactttaaaa ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg
     5101 ctaagggtta atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat
     5161 actattcctt tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg
     5221 ggaccacctt atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct
     5281 cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa
     5341 taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     5401 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     5461 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     5521 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     5581 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     5641 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     5701 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggtgc ggtatttcac
     5761 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5821 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5881 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5941 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6001 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6361 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     6421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7321 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7561 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7741 gatgctcgtc aggggggcgg agcctatgga aaaacgcctt cttcttgaac tcgggctcgg
     7801 tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc
     7861 tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca
     7921 ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc
     7981 tgccgctgga ctacgtgggt ctggcc
//
