pFosill-2 vector (V006201)

LOCUS       40924_20461        9902 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9902)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9902)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2012"; volume: "22"; issue: "11"; pages: "2241-2249"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and Harvard, 320 
            Charles Street, Cambridge, MA 02141, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..50
                     /note="SBS12 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
     promoter        complement(90..107)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      91..107
                     /note="SP6 promoter; transcript counterclockwise"
                     /regulatory_class="promoter"
     CDS             complement(250..906)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(907..1009)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1848..1914
                     /note="ori2; oriS"
     rep_origin      1933..2152
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2243..2995
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    3001..3251
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3577..4749
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             4752..5720
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    5796..6269
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6528..7198
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6529..6928
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7335..7439
                     /label=AmpR promoter
     CDS             7440..8297
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8471..9059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9330..9728)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9746..9779)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9795..9813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9854..9886
                     /note="SBS3 Illumina sequence; designed for amplification
                     of Illumina fosmid library"