LOCUS       40924_20461        9902 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFosill-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Williams LJ, Tabbaa DG, Li N, Berlin AM, Shea TP, Maccallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Paired-end sequencing of Fosmid libraries by Illumina
  JOURNAL   Genome Res. 22 (11), 2241-2249 (2012)
  PUBMED    22800726
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Williams LJS., Tabbaa DG, Li N, Berlin AM, Shea TP, MacCallum I, 
            Lawrence MS, Drier Y, Getz G, Young SK, Jaffe DB, Nusbaum C, Gnirke 
            A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and 
            Harvard, 320 Charles Street, Cambridge, MA 02141, USA
REFERENCE   3  (bases 1 to 9902)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9902)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2012"; volume: "22"; issue: "11"; pages: "2241-2249"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-MAY-2012) MBRD, GSAP, Broad Institute of MIT and Harvard, 320 
            Charles Street, Cambridge, MA 02141, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..50
                     /note="SBS12 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
     promoter        complement(90..107)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      91..107
                     /note="SP6 promoter; transcript counterclockwise"
                     /regulatory_class="promoter"
     CDS             complement(250..906)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(907..1009)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      1848..1914
                     /note="ori2; oriS"
     rep_origin      1933..2152
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             2243..2995
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    3001..3251
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3577..4749
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             4752..5720
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    5796..6269
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    6528..7198
                     /label=lambda cos site
                     /note="lambda cos site"
     misc_feature    6529..6928
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        7335..7439
                     /label=AmpR promoter
     CDS             7440..8297
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8471..9059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9330..9728)
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(9746..9779)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9795..9813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9854..9886
                     /note="SBS3 Illumina sequence; designed for amplification
                     of Illumina fosmid library"
ORIGIN
        1 tccagcatgc acgtggagat cggaagagca cacgtctgaa ctccagtcac atcctcagca
       61 gtgtcgtatg ccgtcttctg cttgagatcc tatagtgtca cctaaatcgt atgcggccgc
      121 ccgggccgtc gaccaattct catgtttgac agcttatcat cgaatttctg ccattcatcc
      181 gcttattatc acttattcag gcgtagcacc aggcgtttaa gggcaccaat aactgcctta
      241 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      301 gccgacatgg aagccatcac agacggcatg atgaacctga atcgccagcg gcatcagcac
      361 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      421 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
      481 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      541 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      601 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      661 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
      721 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      781 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      841 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      901 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      961 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     1021 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     1081 tgcgaagtga tcttccgtca caggtattta ttcgcgataa gctcatggag cggcgtaacc
     1141 gtcgcacagg aaggacagag aaagcgcgga tctgggaagt gacggacaga acggtcagga
     1201 cctggattgg ggaggcggtt gccgccgctg ctgctgacgg tgtgacgttc tctgttccgg
     1261 tcacaccaca tacgttccgc cattcctatg cgatgcacat gctgtatgcc ggtataccgc
     1321 tgaaagttct gcaaagcctg atgggacata agtccatcag ttcaacggaa gtctacacga
     1381 aggtttttgc gctggatgtg gctgcccggc accgggtgca gtttgcgatg ccggagtctg
     1441 atgcggttgc gatgctgaaa caattatcct gagaataaat gccttggcct ttatatggaa
     1501 atgtggaact gagtggatat gctgtttttg tctgttaaac agagaagctg gctgttatcc
     1561 actgagaagc gaacgaaaca gtcgggaaaa tctcccatta tcgtagagat ccgcattatt
     1621 aatctcagga gcctgtgtag cgtttatagg aagtagtgtt ctgtcatgat gcctgcaagc
     1681 ggtaacgaaa acgatttgaa tatgccttca ggaacaatag aaatcttcgt gcggtgttac
     1741 gttgaagtgg agcggattat gtcagcaatg gacagaacaa cctaatgaac acagaaccat
     1801 gatgtggtct gtccttttac agccagtagg ctcgccgcag tcgagcgacg gcgaagccct
     1861 cgagtgagcg aggaagcacc agggaacagc acttatatat tctgcttaca cacgatgcct
     1921 gaaaaaactt cccttggggt tatccactta tccacgggga tatttttata attatttttt
     1981 ttatagtttt tagatcttct tttttagagc gccttgtagg cctttatcca tgctggttct
     2041 agagaaggtg ttgtgacaaa ttgccctttc agtgtgacaa atcaccctca aatgacagtc
     2101 ctgtctgtga caaattgccc ttaaccctgt gacaaattgc cctcagaaga agctgttttt
     2161 tcacaaagtt atccctgctt attgactctt ttttatttag tgtgacaatc taaaaacttg
     2221 tcacacttca catggatctg tcatggcgga aacagcggtt atcaatcaca agaaacgtaa
     2281 aaatagcccg cgaatcgtcc agtcaaacga cctcactgag gcggcatata gtctctcccg
     2341 ggatcaaaaa cgtatgctgt atctgttcgt tgaccagatc agaaaatctg atggcaccct
     2401 acaggaacat gacggtatct gcgagatcca tgttgctaaa tatgctgaaa tattcggatt
     2461 gacctctgcg gaagccagta aggatatacg gcaggcattg aagagtttcg cggggaagga
     2521 agtggttttt tatcgccctg aagaggatgc cggcgatgaa aaaggctatg aatcttttcc
     2581 ttggtttatc aaacgtgcgc acagtccatc cagagggctt tacagtgtac atatcaaccc
     2641 atatctcatt cccttcttta tcgggttaca gaaccggttt acgcagtttc ggcttagtga
     2701 aacaaaagaa atcaccaatc cgtatgccat gcgtttatac gaatccctgt gtcagtatcg
     2761 taagccggat ggctcaggca tcgtctctct gaaaatcgac tggatcatag agcgttacca
     2821 gctgcctcaa agttaccagc gtatgcctga cttccgccgc cgcttcctgc aggtctgtgt
     2881 taatgagatc aacagcagaa ctccaatgcg cctctcatac attgagaaaa agaaaggccg
     2941 ccagacgact catatcgtat tttccttccg cgatatcact tccatgacga caggatagtc
     3001 tgagggttat ctgtcacaga tttgagggtg gttcgtcaca tttgttctga cctactgagg
     3061 gtaatttgtc acagttttgc tgtttccttc agcctgcatg gattttctca tactttttga
     3121 actgtaattt ttaaggaagc caaatttgag ggcagtttgt cacagttgat ttccttctct
     3181 ttcccttcgt catgtgacct gatatcgggg gttagttcgt catcattgat gagggttgat
     3241 tatcacagtt tattactctg aattggctat ccgcgtgtgt acctctacct ggagtttttc
     3301 ccacggtgga tatttcttct tgcgctgagc gtaagagcta tctgacagaa cagttcttct
     3361 ttgcttcctc gccagttcgc tcgctatgct cggttacacg gctgcggcga gcgctagtga
     3421 taataagtga ctgaggtatg tgctcttctt atctcctttt gtagtgttgc tcttatttta
     3481 aacaactttg cggttttttg atgactttgc gattttgttg ttgctttgca gtaaattgca
     3541 agatttaata aaaaaacgca aagcaatgat taaaggatgt tcagaatgaa actcatggaa
     3601 acacttaacc agtgcataaa cgctggtcat gaaatgacga aggctatcgc cattgcacag
     3661 tttaatgatg acagcccgga agcgaggaaa ataacccggc gctggagaat aggtgaagca
     3721 gcggatttag ttggggtttc ttctcaggct atcagagatg ccgagaaagc agggcgacta
     3781 ccgcacccgg atatggaaat tcgaggacgg gttgagcaac gtgttggtta tacaattgaa
     3841 caaattaatc atatgcgtga tgtgtttggt acgcgattgc gacgtgctga agacgtattt
     3901 ccaccggtga tcggggttgc tgcccataaa ggtggcgttt acaaaacctc agtttctgtt
     3961 catcttgctc aggatctggc tctgaagggg ctacgtgttt tgctcgtgga aggtaacgac
     4021 ccccagggaa cagcctcaat gtatcacgga tgggtaccag atcttcatat tcatgcagaa
     4081 gacactctcc tgcctttcta tcttggggaa aaggacgatg tcacttatgc aataaagccc
     4141 acttgctggc cggggcttga cattattcct tcctgtctgg ctctgcaccg tattgaaact
     4201 gagttaatgg gcaaatttga tgaaggtaaa ctgcccaccg atccacacct gatgctccga
     4261 ctggccattg aaactgttgc tcatgactat gatgtcatag ttattgacag cgcgcctaac
     4321 ctgggtatcg gcacgattaa tgtcgtatgt gctgctgatg tgctgattgt tcccacgcct
     4381 gctgagttgt ttgactacac ctccgcactg cagtttttcg atatgcttcg tgatctgctc
     4441 aagaacgttg atcttaaagg gttcgagcct gatgtacgta ttttgcttac caaatacagc
     4501 aatagtaatg gctctcagtc cccgtggatg gaggagcaaa ttcgggatgc ctggggaagc
     4561 atggttctaa aaaatgttgt acgtgaaacg gatgaagttg gtaaaggtca gatccggatg
     4621 agaactgttt ttgaacaggc cattgatcaa cgctcttcaa ctggtgcctg gagaaatgct
     4681 ctttctattt gggaacctgt ctgcaatgaa attttcgatc gtctgattaa accacgctgg
     4741 gagattagat aatgaagcgt gcgcctgtta ttccaaaaca tacgctcaat actcaaccgg
     4801 ttgaagatac ttcgttatcg acaccagctg ccccgatggt ggattcgtta attgcgcgcg
     4861 taggagtaat ggctcgcggt aatgccatta ctttgcctgt atgtggtcgg gatgtgaagt
     4921 ttactcttga agtgctccgg ggtgatagtg ttgagaagac ctctcgggta tggtcaggta
     4981 atgaacgtga ccaggagctg cttactgagg acgcactgga tgatctcatc ccttcttttc
     5041 tactgactgg tcaacagaca ccggcgttcg gtcgaagagt atctggtgtc atagaaattg
     5101 ccgatgggag tcgccgtcgt aaagctgctg cacttaccga aagtgattat cgtgttctgg
     5161 ttggcgagct ggatgatgag cagatggctg cattatccag attgggtaac gattatcgcc
     5221 caacaagtgc ttatgaacgt ggtcagcgtt atgcaagccg attgcagaat gaatttgctg
     5281 gaaatatttc tgcgctggct gatgcggaaa atatttcacg taagattatt acccgctgta
     5341 tcaacaccgc caaattgcct aaatcagttg ttgctctttt ttctcacccc ggtgaactat
     5401 ctgcccggtc aggtgatgca cttcaaaaag cctttacaga taaagaggaa ttacttaagc
     5461 agcaggcatc taaccttcat gagcagaaaa aagctggggt gatatttgaa gctgaagaag
     5521 ttatcactct tttaacttct gtgcttaaaa cgtcatctgc atcaagaact agtttaagct
     5581 cacgacatca gtttgctcct ggagcgacag tattgtataa gggcgataaa atggtgctta
     5641 acctggacag gtctcgtgtt ccaactgagt gtatagagaa aattgaggcc attcttaagg
     5701 aacttgaaaa gccagcaccc tgatgcgacc acgttttagt ctacgtttat ctgtctttac
     5761 ttaatgtcct ttgttacagg ccagaaagca taactggcct gaatattctc tctgggccca
     5821 ctgttccact tgtatcgtcg gtctgataat cagactggga ccacggtccc actcgtatcg
     5881 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
     5941 ctgggaccac ggtcccactc gtatcgtcgg tctgataatc agactgggac cacggtccca
     6001 ctcgtatcgt cggtctgatt attagtctgg gaccatggtc ccactcgtat cgtcggtctg
     6061 attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctggaacc
     6121 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc
     6181 gtcggtctga ttattagtct gggaccacga tcccactcgt gttgtcggtc tgattatcgg
     6241 tctgggacca cggtcccact tgtattgtcg atcagactat cagcgtgaga ctacgattcc
     6301 atcaatgcct gtcaagggca agtattgaca tgtcgtcgta acctgtagaa cggagtaacc
     6361 tcggtgtgcg gttgtatgcc tgctgtggat tgctgctgtg tcctgcttat ccacaacatt
     6421 ttgcgcacgg ttatgtggac aaaatacctg gttacccagg ccgtgccggc acgttaaccg
     6481 ggctgcatcc gatgcaagtg tgtcgctgtc gacgagctcg cgagctcgga catgaggttg
     6541 ccccgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt aagttgatgc
     6601 agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt gatggcctcc
     6661 acgcacgttg tgatatgtag atgataatca ttatcacttt acgggtcctt tccggtgatc
     6721 cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat tttccggttt
     6781 aaggcgtttc cgttcttctt cgtcataact taatgttttt atttaaaata ccctctgaaa
     6841 agaaaggaaa cgacaggtgc tgaaagcgag gctttttggc ctctgtcgtt tcctttctct
     6901 gtttttgtcc gtggaatgaa caatggaagt cctggcgtaa tagcgaagag gcccgcaccg
     6961 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     7021 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     7081 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7141 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7201 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7261 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7321 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7381 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7441 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7501 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7561 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7621 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7681 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7741 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7801 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     7861 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7921 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7981 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8041 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8101 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8161 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8221 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8281 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8341 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8401 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8461 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8521 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8581 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     8641 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8701 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8761 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8821 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     8881 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8941 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9001 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9061 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9121 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9181 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9241 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcttgc
     9301 gccgttccac ttcgatgcgt cagtgaagcg acatgaggtt gccccgtatt cagtgtcgct
     9361 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
     9421 ctgcgtcata attgattatt tgacgtggtt tgatggcctc cacgcacgtt gtgatatgta
     9481 gatgataatc attatcactt tacgggtcct ttccggtgat ccgacaggtt acggggcggc
     9541 gacctcgcgg gttttcgcta tttatgaaaa ttttccggtt taaggcgttt ccgttcttct
     9601 tcgtcataac ttaatgtttt tatttaaaat accctctgaa aagaaaggaa acgacaggtg
     9661 ctgaaagcga gctttttggc ctctgtcgtt tcctttctct gtttttgtcc gtggaatgaa
     9721 caatggaagt ccgagctcat cgctaataac ttcgtatagc atacattata cgaagttata
     9781 ttcgatgcgg ccgctaatac gactcactat agggagaagc ttaatgatac ggcgaccacc
     9841 gacactgctg aggacactct ttccctacac gacgctcttc cgatctccac gtgcatgctg
     9901 ga
//
