pFNK-101 vector (V006210)

LOCUS       40924_20411        4099 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFNK-101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Basu S, Gerchman Y, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2005) Electrical Engineering, Princeton 
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4099)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4099)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2005) Electrical Engineering, Princeton University, B-312, 
            E-Quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(79..1158)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1159..1236)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1302..1323
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1338..1368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1376..1392
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1403..1414
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1421..2104
                     /label=HcRed1
                     /note="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
     terminator      2186..2272
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2442..2987)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3101..3195)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3229..4020)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"