LOCUS       40924_20411        4099 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFNK-101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Basu S, Gerchman Y, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2005) Electrical Engineering, Princeton 
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4099)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4099)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2005) Electrical Engineering, Princeton University, B-312, 
            E-Quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(79..1158)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1159..1236)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1302..1323
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1338..1368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1376..1392
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1403..1414
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1421..2104
                     /label=HcRed1
                     /note="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
     terminator      2186..2272
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2442..2987)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3101..3195)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3229..4020)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccgac
       61 gtcattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      121 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
      181 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
      241 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
      301 tgacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
      361 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
      421 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
      481 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
      541 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
      601 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
      661 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
      721 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
      781 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
      841 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
      901 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
      961 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     1021 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     1081 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     1141 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     1201 cataccgcga aaggttttgc accattcgat ggtgtcaacg taatctagag ctagcacgac
     1261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1381 agcggataac aatttcacaa gcaaagagga gaaattaagc atggtgagcg gcctgctgaa
     1441 ggagagtatg cgcatcaaga tgtacatgga gggcaccgtg aacggccact acttcaagtg
     1501 cgagggcgag ggcgacggca accccttcgc cggcacccag agcatgagaa tccacgtgac
     1561 cgagggcgcc cccctgccct tcgccttcga catcctggcc ccctgctgcg agtacggcag
     1621 caggaccttc gtgcaccaca ccgccgagat ccccgacttc ttcaagcaga gcttccccga
     1681 gggcttcacc tgggagagaa ccaccaccta cgaggacggc ggcatcctga ccgcccacca
     1741 ggacaccagc ctggagggca actgcctgat ctacaaggtg aaggtgcacg gcaccaactt
     1801 ccccgccgac ggccccgtga tgaagaacaa gagcggcggc tgggagccca gcaccgaggt
     1861 ggtgtacccc gagaacggcg tgctgtgcgg ccggaacgtg atggccctga aggtgggcga
     1921 ccggcacctg atctgccacc actacaccag ctaccggagc aagaaggccg tgcgcgccct
     1981 gaccatgccc ggcttccact tcaccgacat ccggctccag atgctgcgga agaagaagga
     2041 cgagtacttc gagctgtacg aggccagcgt ggcccggtac agcgacctgc ccgagaaggc
     2101 caactgagtt cacgaaaact acgctttagt agcttaataa gcttaattag ctgacctact
     2161 agtcggccgt ctcgagtaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2221 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     2281 ctagacctag gggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg
     2341 actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa
     2401 gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc
     2461 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2521 taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     2581 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     2641 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     2761 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     2821 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     2881 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     2941 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     3001 ttaatcagat aaaatattac tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     3061 aagtcagccc catacgatat aagttgttac tagtgcttgg attctcacca ataaaaaacg
     3121 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     3181 ctatcaacag gagtccaagc gagctctcga accccagagt cccgctcaga agaactcgtc
     3241 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     3301 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     3361 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     3421 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     3481 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     3541 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     3601 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     3661 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     3721 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     3781 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     3841 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     3901 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     3961 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     4021 gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct
     4081 tggcggcaag aaagccatc
//
