pFM141 vector (V006218)

LOCUS       V006218                 8180 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006218
VERSION     V006218
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
   PUBMED   24032656
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 8180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..850
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      1011..1070
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     terminator      1130..1159
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     terminator      complement(1288..1317)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    1364..1385
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             complement(1389..2264)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        2291..2575
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_feature    2604..2616
                     /label="RBS B0032"
                     /note="RBS B0032"
     CDS             2623..3684
                     /gene="ada"
                     /label="Bifunctional transcriptional activator/DNA repair
                     enzyme Ada"
                     /note="Bifunctional transcriptional activator/DNA repair
                     enzyme Ada from Escherichia coli (strain K12). Accession#:
                     P06134"
     misc_feature    3688..3708
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      3744..3775
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             4662..5630
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     rep_origin      5670..6105
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     rep_origin      complement(7638..8026)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"