LOCUS       V006218                 8180 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006218
VERSION     V006218
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
   PUBMED   24032656
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 8180)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8180)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..850
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      1011..1070
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     terminator      1130..1159
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     terminator      complement(1288..1317)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    1364..1385
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             complement(1389..2264)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
     promoter        2291..2575
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     misc_feature    2604..2616
                     /label="RBS B0032"
                     /note="RBS B0032"
     CDS             2623..3684
                     /gene="ada"
                     /label="Bifunctional transcriptional activator/DNA repair
                     enzyme Ada"
                     /note="Bifunctional transcriptional activator/DNA repair
                     enzyme Ada from Escherichia coli (strain K12). Accession#:
                     P06134"
     misc_feature    3688..3708
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      3744..3775
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             4662..5630
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     rep_origin      5670..6105
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     rep_origin      complement(7638..8026)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
       61 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
      121 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
      181 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
      241 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
      301 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
      361 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
      421 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
      481 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
      541 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
      601 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
      661 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
      721 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
      781 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
      841 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
      901 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
      961 gcttttatta tttttaagcg tgcataataa gccctacaca aatggtaccc tccggcaaaa
     1021 aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg
     1081 tttgccacct gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc
     1141 ccacccgtga aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt
     1201 gactcgaatt cgcggccgct gcccgatcaa ctcgcgcgtt tgccacctga cgtctaagaa
     1261 aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag gtgagccagt
     1321 gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg cggccgcttc
     1381 tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac
     1441 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
     1501 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
     1561 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
     1621 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca
     1681 aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc
     1741 atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc
     1801 agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc
     1861 ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg
     1921 gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc
     1981 cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc
     2041 gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc
     2101 ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata
     2161 aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta
     2221 aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg
     2281 ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt
     2341 tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa
     2401 agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa
     2461 gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata
     2521 agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg
     2581 tttttttggg ctagctacta gagtcacaca ggaaagtact agatgaaaaa agccacatgc
     2641 ttaactgacg atcaacgctg gcaatctgtc ttagcccgcg acccgaatgc cgacggcgaa
     2701 ttcgttttcg ccgtgcgtac cacaggcatc ttttgccgtc cgtcttgccg cgccagacat
     2761 gctttgcggg aaaacgtctc cttctacgca aatgccagcg aggcactcgc cgctggcttt
     2821 cgcccctgca aacgttgtca gccagaaaaa gccaatgccc agcaacatcg gttggataaa
     2881 atcacccacg cgtgtcgact gctggaacag gaaacgcctg taacgctgga agccttagcc
     2941 gaccaggtgg cgatgagtcc atttcatcta catcggttgt ttaaagcgac taccggaatg
     3001 acgcctaaag cctggcaaca ggcctggcgc gctcgccgtt tgcgcgaatc gctggcgaaa
     3061 ggggagagcg tgacgacgtc tattcttaac gccggattcc ccgacagcag cagttactat
     3121 cgcaaagctg acgaaacgct gggcatgacg gctaaacaat tccgtcacgg tggcgaaaat
     3181 ctggcggtgc gttacgcgct ggctgattgt gagctgggtc gttgcctggt ggcagaaagc
     3241 gagcggggga tttgcgcgat attgctgggc gatgatgacg cgacactaat cagcgagttg
     3301 cagcagatgt ttcccgctgc cgacaacgcg cctgccgatc tgatgtttca gcaacatgtg
     3361 cgtgaagtga tcgccagcct caatcaacgc gatacgccgc tgacgttacc gctggacatt
     3421 cgcggcactg cttttcagca acaagtctgg caggcactgc gcacgatacc ttgcggtgaa
     3481 accgtcagtt atcagcaact ggctaacgcc atcggcaaac cgaaagcggt acgggccgtt
     3541 gccagcgcct gtgccgccaa caagctggct atcataatac cctgtcatcg ggtggtccgt
     3601 ggtgatggca cactttccgg ttaccgctgg ggcgtgtcgc gtaaagcgca actgctgcgc
     3661 cgcgaagctg aaaatgagga gaggtaatac tagtagcggc cgctgcagga gtcactaagg
     3721 gttagttagt tagattagca gaaagtcaaa agcctccgac cggaggcttt tgactaaaac
     3781 ttcccttggg gttatcattg gggctcactc aaaggcggta atcagatgct cactcaaagg
     3841 cggtaatcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgc tagagctgtc
     3901 agaccagagc tccaaaccaa cgttttatct ataccgctac agggtattta attcctattt
     3961 aatctgcgct agaatgaggc atgtttaacc gaatctgacg ttttccctgc aaatgccaaa
     4021 atactatgcc tatctccggg tttcgcgtga cggccaagac ccggaaaacc aaaaatacgg
     4081 tttgctcgaa tacgcgaacg ccaaaggctt cgcgccgcta cagatcgagg aagaaattgc
     4141 cagcagagca aaggactggc gcaagcgcaa gctcggagca atcatcgaaa aggccgagcg
     4201 tggcgacgtg ctactgacgc cggagattac gcgcattgcc ggttccgccc tcgccgcctt
     4261 ggaaattctc aaagcggcga gcgagcgcgg cctaatcgtc catgtgacca aacagaagat
     4321 catcatggac ggcagcctac aaagcgacat catggcaacc gtgcttggct tggctgcaca
     4381 gatcgagcgg catttcattc aggcacgtac caccgaggcg ctacaagtcg ccagagagcg
     4441 cggcaagacg ctcgggcgac ccaagggcag caaatcgagc gccttgaagc tggacagccg
     4501 tattgatgaa gtacaggcat acgtgaacct tggcttgccg caaagtcgcg cagccgagtt
     4561 gttaggcgtc agccctcaca ccttgcgcct gttcatcaaa cgccggaaca tcaaacccac
     4621 aaacactaga ccaaccatca ccatgccggg gagggaacaa catgcctaag aacaacaaag
     4681 cccccggcca tcgtatcaac gagatcatca agacgagcct cgcgctcgaa atggaggatg
     4741 cccgcgaagc tggcttagtc ggctacatgg cccgttgcct tgtgcaagcg accatgcccc
     4801 acaccgaccc caagaccagc tactttgagc gcaccaatgg catcgtcacc ttgtcgatca
     4861 tgggcaagcc gagcatcggc ctgccctacg gttctatgcc gcgcaccttg cttgcttgga
     4921 tatgcaccga ggccgtgcga acgaaagacc ccgtgttgaa ccttggccgg tcgcaatcgg
     4981 aatttctaca aaggctcgga atgcacaccg atggccgtta cacggccacc cttcgcaatc
     5041 aggcgcaacg cctgttttca tccatgattt cgcttgccgg cgagcaaggc aatgacttcg
     5101 gcattgagaa cgtcgtcatt gccaagcgcg cttttctatt ctggaatccc aagcggccag
     5161 aagatcgggc gctatgggat agcaccctca ccctcacagg cgatttcttc gaggaagtca
     5221 cccgctcacc ggttcctatc cgaatcgact acctgcatgc cttgcggcag tctccgcttg
     5281 cgatggacat ttacacgtgg ctgacctatc gcgtgttcct gttgcgggcc aagggccgcc
     5341 ccttcgtgca aatcccttgg gtcgccctgc aagcgcaatt cggctcatcc tatggcagcc
     5401 gcgcacgcaa ctcgcccgaa ctggacgata aggcccgaga gcgggcagag cgggcagcac
     5461 tcgccagctt caaatacaac ttcaaaaagc gcctacgcga agtgttgatt gtctatcccg
     5521 aggcaagcga ctgcatcgaa gatgacggcg aatgcctgcg catcaaatcc acacgcctgc
     5581 atgtcacccg cgcacccggc aagggcgctc gcatcggccc ccctccgact tgaccaggcc
     5641 aacgctacgc ttggcttggt caagccttcc catccaacag cccgccgtcg agcgggcttt
     5701 tttatccccg gaagcctgtg gatagagggt agttatccac gtgaaaccgc taatgccccg
     5761 caaagccttg attcacgggg ctttccggcc cgctccaaaa actatccacg tgaaatcgct
     5821 aatcagggta cgtgaaatcg ctaatcggag tacgtgaaat cgctaataag gtcacgtgaa
     5881 atcgctaatc aaaaaggcac gtgagaacgc taatagccct ttcagatcaa cagcttgcaa
     5941 acacccctcg ctccggcaag tagttacagc aagtagtatg ttcaattagc ttttcaatta
     6001 tgaatatata tatcaattat tggtcgccct tggcttgtgg acaatgcgct acgcgcaccg
     6061 gctccgcccg tggacaaccg caagcggttg cccaccgtcg agcgccagcg cctttgccca
     6121 caacccggcg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
     6181 gaaaggcggc aacctctcgg gcttctggat ttccgatcaa cgcaggagtc gttcggaaag
     6241 tagctgttcc agaattatag gcgcagagac accagattcc aagatggctc tgttaaattg
     6301 ttgtagtatg tagtatcata caacatacta cagtacagag gcccgcaaga atggcaatca
     6361 ctaaacaaga catttggcga gcagccgacg aactggacgc cgaaggcatc cggcccactt
     6421 tggccgccgt gcgcaagaaa ctcggaagcg gtagcttcac aaccatttcc gatgcaatgg
     6481 ctgaatggaa aaaccgcaag accgccaccc tgccctcatc agacccattg ccggttgcag
     6541 tcaacgagca tcttgccgag cttggcaatg cgctatgggc tatcgccctg gcgcacgcca
     6601 acgcccggtt tgacgaagat cggaaacaga tcgaggccga caaagcggcc atcagccagc
     6661 agcttgccga agcaatcgaa ctagccgaca ccttcacccg cgaaaacgac cagctccgcg
     6721 aacgagtgaa tcagctcgaa cctatggaac gcgagcgcga caagctggcc gaccagcttg
     6781 ccgaagtgaa acgccgcagc ggcgaagaac taaaccgctg catggaaaag ctcacccaac
     6841 gcgataacga ggctatcgag gcccgcaaac aggccaagga ggccatcgag cgcgccgcca
     6901 gtctgcaagg tcaggtggaa gccctcaaag agcaggtcgc caatctcaca gccgtcttga
     6961 aaacaggagg caaacaatga aaagcgccct tgccgccctt cgcgcggtcg cggccgctgt
     7021 cgtcctaatc gtcagtgtgc ccgcttgggc cgacttccgg ggtgaagtcg tccgaatcct
     7081 tgacggtgac actatcgacg ttttggtgaa ccgtcagacc atccgcgtga gattggccga
     7141 tattgacgca ccggaaagcg gccaagcctt cggctcccgt gctcgccaac ggctcgccga
     7201 cttgaccttt cgccaagagg ttcaagtgac cgaaaaagag gttgatcggt atggccgcac
     7261 tcttggggtc gtttacgcgc cgttgcaata ccccggcggc caaacacaac tcaccaacat
     7321 caatgcgatc atggttcaag aaggcatggc ctgggcttac cgttattacg gcaaaccaac
     7381 cgacgcgcag atgtacgagt atgaaaaaga ggcccgccgc caacggctcg gcctttggtc
     7441 agacccgaat gctcaggagc cttggaaatg gcgtcgcgcc tcgaaaaatg ccacgaactg
     7501 acaccgggca cgccccttgt tcgacgcgcc gcaggcacgt cgaatttacc gccgggacgc
     7561 ccctcgtccc gacacttcca gatcgccata gcgcacagcg cctcgagtga ttcgcacggg
     7621 cccatggcta attcccatgt cagccgttaa gtgttcctgt gtcactcaaa attgctttga
     7681 gaggctctaa gggcttctca gtgcgttaca tccctggctt gttgtccaca accgttaaac
     7741 cttaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta aaaccttaga
     7801 ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag cttagtacgt
     7861 gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca tgagggttta
     7921 gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt gaaacatgag
     7981 agcttagtac gtactatcaa caggttgaac tgctgatctt cagatcctct acgccggacg
     8041 catcgtggcc ggatcttctc gagcggtggt aacggcgcag tggcggtttt catggcttgt
     8101 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     8161 gccgtgggtc gatgtttgat
//
