pFLAG97-TSR vector (V006235)

LOCUS       40924_20161        6116 bp DNA     circular SYN 18-DEC-2018
DEFINITION  FLAG-tagging vector pFLAG97-TSR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6116)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "19"; pages: "10659-10668"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2007) Biological Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1017..1035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1057..1375
                     /label=UL96 derived from human cytomegalovirus
                     /note="UL96 derived from human cytomegalovirus"
     CDS             1499..1522
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3081..3125
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    3126..3143
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             3267..4079
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    4113..4157
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    4769..4785
                     /note="UL96 derived from human cytomegalovirus; alkaline 
                     exonuclease"
     promoter        complement(4787..4805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        5151..5255
                     /label=AmpR promoter
     CDS             5256..6113
                     /label=AmpR
                     /note="beta-lactamase"