LOCUS       40924_20161        6116 bp DNA     circular SYN 18-DEC-2018
DEFINITION  FLAG-tagging vector pFLAG97-TSR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Human cytomegalovirus protein kinase UL97 forms a complex with the 
            tegument phosphoprotein pp65
  JOURNAL   J. Virol. 81 (19), 10659-10668 (2007)
  PUBMED    17634236
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Kamil JP, Coen DM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6116)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6116)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "19"; pages: "10659-10668"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2007) Biological Chemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1017..1035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1057..1375
                     /label=UL96 derived from human cytomegalovirus
                     /note="UL96 derived from human cytomegalovirus"
     CDS             1499..1522
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    3081..3125
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    3126..3143
                     /label=I-SceI homing endonuclease recognition site
                     /note="I-SceI homing endonuclease recognition site"
     CDS             3267..4079
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    4113..4157
                     /note="45-bp repeat; Red recombination substrate"
     misc_feature    4769..4785
                     /note="UL96 derived from human cytomegalovirus; alkaline 
                     exonuclease"
     promoter        complement(4787..4805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        5151..5255
                     /label=AmpR promoter
     CDS             5256..6113
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      961 cctctccccg cgcgttggcc gattcattaa tgcaggttaa cctggcttat cgaaattaat
     1021 acgactcact atagggagac cggcagatct gatatctcag tttctgcggc gtacctacgg
     1081 accgcagcac cggctcacca cgcagcaggc tttgacggtg atgcgtgtgg ccgctcggga
     1141 acagacccga tacagtcagc gaacgacgca gtgcgtggcc gcacacctgt tggagcaacg
     1201 ggcggccgtg cagcaagagt tgcaacgcgc ccgacagctg caatccggta acgtggacga
     1261 cgcgctggac tctttaaccg agctgaagga cacggtagac gatgtgagag ccaccttggt
     1321 ggactcggtt tcggcgacgt gcgatttgga cctggaggtc gacgacgccg tctaacaggt
     1381 atagcaatct ccgtcacgcc tctgttcaga ttttattaaa aaaaaaacac aacataacga
     1441 cagtgtcggt gtggtagcta gtgcagcctt aggaacaggg aagactgtcg ccactatgga
     1501 ctacaaggat gacgacgata agtcctccgc acttcggtct cgggctcgct cggcctcgct
     1561 cggaacgacg actcagggct gggatccgcc gccattgcgt cgtcccagca gggcgcgccg
     1621 gcgccagtgg atgcgcgaag ctgcgcaggc cgccgctcaa gccgcggtgc aggccgcgca
     1681 ggccgccgcc gctcaggtcg cccaggctca cgttgatgaa aacgaggtcg tggatctgat
     1741 ggccgacgag gccggcggcg gcgtcaccac tttgaccacc ctgagttccg tcagcacaac
     1801 caccgtgctt ggacacgcga ctttttccgc atgcgttcga agtgacgtga tgcgtgacgg
     1861 agaaaaagag gacgcggctt cggacaagga gaacctgcgt cggcccgtag tgccgtccac
     1921 gtcgtctcgc ggcagcgccg ccagcggcga cggttaccac ggcttgcgct gccgcgaaac
     1981 ttcggccatg tggtcgttcg agtacgatcg cgacggcgac gtgaccagcg tacgccgcgc
     2041 tctcttcacc ggcggcagcg acccctcgga cagcgtgagc ggcgtccgcg gtggacgcaa
     2101 acgcccgttg cgtccgccgt tggtgtcgct ggcccgcacc ccgctgtgcc gacgtcgtgt
     2161 gggcggtgtg gacgcggtgc tcgaagaaaa cgacgtggag ctgcgcgcgg aaagtcagga
     2221 cagcgccgtg gcatcgggcc cgggccgcat tccgcagccg ctcagcggta gttccgggga
     2281 ggaatccgcc acggcggtgg aggccgactc cacgtcacac gacgacgtgc attgcacctg
     2341 ttccaacgac cagatcatca ccacgtccat ccgcggcctt acgtgcgacc cgcgtatgtt
     2401 cttgcgcctt acgcatcccg agctctgcga gctctctatc tcctacctgc tggtctacgt
     2461 gcccaaagag gacgattttt gccacaagat ttgttatgcc gtggacatga gcgacgagag
     2521 ctaccgcctg ggccagggct ccttcggcga ggtctggccg ctcgatcgct atcgcgtggt
     2581 caaggtggcg cgtaagcaca gcgagacggt gctcacggtc tggatgtcgg gcctgatccg
     2641 cacgcgcgcc gctggcgagc aacagcagcc gccgtcgctg gtgggcacgg gcgtgcaccg
     2701 cggtctgctc acggccacgg gctgctgtct gctgcacaac gtcacggtac atcgacgttt
     2761 ccacacagac atgtttcatc acgaccagtg gaagctggcg tgcatcgaca gctaccgacg
     2821 tgccttttgc acgttggccg acgctatcaa atttctcaat caccagtgtc gtgtatgcca
     2881 ctttgacatt acacccatga acgtgctcat cgacgtgaac ccgcacaacc ccagcgagat
     2941 cgtgcgcgcc gcgctgtgcg attacagcct cagcgagccc tatccggatt acaacgagcg
     3001 ctgtgtggcc gtctttcagg agacgggtac ggcgcgccgc atccccaact gctcgcaccg
     3061 tctgcgcgaa tgttaccacc ctgctttccg acccatgccg ctgcagaagc tgctcatctg
     3121 cgacctaggg ataacagggt aatcgattta ttcaacaaag ccacgttgtg tctcaaaatc
     3181 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     3241 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
     3301 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     3361 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     3421 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     3481 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     3541 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     3601 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     3661 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     3721 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     3781 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     3841 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     3901 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     3961 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     4021 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     4081 aatcagaatt ggttaattgg ttgtaacact ggctgctttc cgacccatgc cgctgcagaa
     4141 gctgctcatc tgcgacccgc acgcgcgttt ccccgtagcc ggcctacggc gttattgcat
     4201 gtcggagctg tcggcgctgg gtaacgtgct gggcttttgc ctcatgcggc tgttggaccg
     4261 gcgcggtctg gacgaggtgc gcatgggcac ggaggcgttg ctctttaagc acgccggcgc
     4321 ggcctgccgc gcgttggaga acggtaagct cacgcactgc tccgacgcct gtctgctcat
     4381 tctggcggcg caaatgagct acggcgcctg tctcctgggc gagcatggcg ccgcgctggt
     4441 gtcgcacacg ctgcgctttg tggaggccaa gatgtcctcg tgtcgcgtac gcgcctttcg
     4501 ccgcttctac cacgaatgct cgcagaccat gctgcacgaa tacgtcagaa agaacgtgga
     4561 gcgtctgttg gccacgagcg acgggctgta tttatataac gcctttcggc gcaccaccag
     4621 cataatctgc gaggaggacc ttgacggtga ctgccgccaa ctgttccccg agtaaccggg
     4681 acgcggaacg tgacggttgc tgaggggaaa ggcaacagag aaggtacaaa cccaccggcg
     4741 gggaaaatac cgaggcgccg ccatcatcat gtggggcgtc tcgagttcta tagtgtcacc
     4801 taaatcgtat gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca
     4861 atatgtccat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4921 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4981 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5041 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5101 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5161 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5221 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5461 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5761 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6001 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaa
//
