pFL8 vector (V006238)

LOCUS       V006238                 8921 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006238
VERSION     V006238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Reeves AR, Weber G, Cernota WH, Weber JM.
  TITLE     Analysis of an 8.1-kb DNA fragment contiguous with the erythromycin
            gene cluster of Saccharopolyspora erythraea in the eryCI-flanking
            region
  JOURNAL   Antimicrob. Agents Chemother. 46 (12), 3892-3899 (2002)
   PUBMED   12435693
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Weber JM, Zieler H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2015) Research and Development, Fermalogic, Inc.,
            4222 N. Ravenswood Ave., Suite 208, Chicago, IL 60613, USA
REFERENCE   3  (bases 1 to 8921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "2002"; volume: "46"; issue: "12"; pages:
            "3892-3899"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-APR-2015) Research and Development, Fermalogic, Inc., 4222 N.
            Ravenswood Ave., Suite 208, Chicago, IL 60613, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3426..4232
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             complement(4720..5511)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(5774..6229)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6370..6386
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6393..6411
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6414..6470
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(6477..6495)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6516..6532)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6540..6556)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6564..6594)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6609..6630)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6918..7506)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7680..8537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8538..8642)
                     /label="AmpR promoter"