LOCUS       V006238                 8921 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006238
VERSION     V006238
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Reeves AR, Weber G, Cernota WH, Weber JM.
  TITLE     Analysis of an 8.1-kb DNA fragment contiguous with the erythromycin
            gene cluster of Saccharopolyspora erythraea in the eryCI-flanking
            region
  JOURNAL   Antimicrob. Agents Chemother. 46 (12), 3892-3899 (2002)
   PUBMED   12435693
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Weber JM, Zieler H.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2015) Research and Development, Fermalogic, Inc.,
            4222 N. Ravenswood Ave., Suite 208, Chicago, IL 60613, USA
REFERENCE   3  (bases 1 to 8921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "2002"; volume: "46"; issue: "12"; pages:
            "3892-3899"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-APR-2015) Research and Development, Fermalogic, Inc., 4222 N.
            Ravenswood Ave., Suite 208, Chicago, IL 60613, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3426..4232
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             complement(4720..5511)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(5774..6229)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6370..6386
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6393..6411
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6414..6470
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(6477..6495)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6516..6532)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6540..6556)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6564..6594)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6609..6630)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6918..7506)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7680..8537)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8538..8642)
                     /label="AmpR promoter"
ORIGIN
        1 taatgatctg tcgtgcaatg ttgaacaaag atgatcttgc tcgcggcggt cctcgccgac
       61 gggcggaagg ggcaattcct ttccgagcgg acgtcccttc cggtggtctt tggcgcgggt
      121 gcgccccgcc ggtggcgtgg ctcacgtgcg aaaactgacg tgatcacccc cgacagcgga
      181 tcaaggggtt tgcgggtccc ggtcggcgcc gggcggggga ggcaggagcc gccgacgctg
      241 cctctgggac gggccggacg gcagggggac cggcggccgg gcgagctgca gccgggggtc
      301 cggcagggcc ggagcgggcg gaaccgtgct ctgacctgcg gcccgagttt cgtcacgtga
      361 cggaatggaa ggctgctgca tttcgtcacg tgacgtatct cggcgagcga ctgccgacgc
      421 cacggcggac acgatcgcct cgcgctggcg ccgggcctcg tacgcccgct ggcggcagga
      481 gcggcggcag tagtcccggc tccggccgac gccggattgc ttgatctccg agccgcacca
      541 ggcgcagagc ttcgcgccgt cggcgtccct gggggtggtg gtgctcatgg ccgacgaccg
      601 tacgcggcac gtctcgtagc gaggcgagtc gggcgcgagg taccgcctgc acgaagtgcc
      661 ggcggggccg accccgggcg agtaatccca ggattactcc cgcggcttcg accccggccg
      721 ccgtcgccgc gtacgtcacc gacccccgcc gtacgtcacc gggatgacgt acggcggggg
      781 ggagcgagtt agtgcgaagt gggcccactt gcgagccggg cgatgtgccg ggcggcccgc
      841 tcctggcggt cgtcggcgtc gtcgtcctgg tcgtcgtcct gctctcgccg tcggcgtgca
      901 gttgcttcct cgcggcgctg ggcgagggcg gcgagcatgt cggcgtacgc ctcggccacc
      961 tcccccgccg tgagcaccac cactgtgtcg gccgcgtcgg ccagcgccag gacctcccgc
     1021 acccgttcgc ccacggccgc cggatcctcg ttgccgtcct tgccttcggc ggcccgggtc
     1081 gcctcgaggt cgagggcgcg gcgggtgacc gcgtgccatc cgtcctcggt cacggcgacc
     1141 ccggcccgca gctccccgcc gtcggcgtcg gccgccagga gcagatcgag gtcgtcggcc
     1201 tcggtgtcgc cgccgtcgag cccgagcatc tgccgcaggt agcgggtcca ttcgatggcc
     1261 cggcgtcccc gggttgcccg ctcgtactcg tgccagcgcg agaggttcca ctccagcgag
     1321 ccgaccccgg cggcgtcgtc ctcggtcatg ccgccggtca ggtccccgat ccgtccgagg
     1381 agttcgaacg gggcgacgtt cccgccggtc gccgtcttga ggtcggcgcg ggcgagttcg
     1441 agggcgggcg ccttcccgtc ctgggtcttg gcgatgtact cggcgaggtc gttggcgtcg
     1501 cgctcggtct ccagccgctt gaagtcgacg ccgtgccggt cgtcgggcgt gaaggcgggg
     1561 ttgaccttgc gcagggcggc ggtccacacg gaccgccagt gcccctgcca ctcgtcgagc
     1621 gcggcgccgg tcggctcgaa ggtggcgacg atctgcttcg cggaccgctc cccctcggtc
     1681 cggccgccga ccaggacgat cgcgtggatg tgcgggtgcc agccgttgat ctgccccacg
     1741 gtgacttcgg tcgcgcggat catgccgacg tacccgatcc ggtctcggat gccctcgcgg
     1801 tcggcggccc ggtgcccgtc cttggcccgg cgtccggccc acgtgccgcc cgtgatcagt
     1861 cgctggtagg cgcccggccg ccgggggctg tccggcgtct tccgggtgcc ctggagggcg
     1921 tccatgaggt ccgcgagccg gtccgtgtgc ccatggcggg ccgtgaaggt gaccaggtag
     1981 gcggtccccc cgcgcttgat ccactcgacc acggcggcgg tgatctcctc ggcccgcttg
     2041 tgccggatcg tggcggcgca gaccgggcag agccagatcc gcccgcaccg catcaggccc
     2101 aggaccacgg acgttccggc cgccgtctgg gcgacgatca cgccggaggc agggtccatc
     2161 agggcgcggc cgcagccctt gcacgcggcg tccccgctga tccgccacag cgtccggcgg
     2221 cggctgtacc gggcggcttt ccgcagtcgg gcagcctcgg tccgcgacgt gcttcctact
     2281 tcccagaggc tgtcgcctct cgggctctcc ccatccaccc cgtccggaga aaccgcaggt
     2341 cggaggggtg cgggaaactc tgttgtttct ttcccaaggt gttcgctttt gcctcgggcg
     2401 gcatctcgcg tcacacgcgc gatcgcccgc ttcgctgcca tccggcagcg gtctgagcag
     2461 tagatacgcg gccgtttgcc cggtgtgtgg gcaattgcgg tcccgcagtg gcagcggggc
     2521 ccggcgggcc gatctggcaa tgcctcggca tcgctccgta ctctgggcac gagcaacgtt
     2581 cctgtctcgc ccggctaagg ggcgcgagtc tgggagcgga cgggtcggag gtgcgaagtc
     2641 cggcccgttg ctctttggtc tggtgggaat cctggcacca atcgggccag aggttccctc
     2701 cgccactccc gacgcccctt ggggctggtg tgacttggag ggccgaagag agccccgccg
     2761 ggtatccggc ggggctttga cgtgcggtca gtgcgtgtgt cggcgagcga tggccacgag
     2821 gccctggaag ccgagcggtc cggcgaagtc ggcccagtcg caaccgggct cagcgcagtg
     2881 ggcggaccag ccaccgccgt tggggtcctg gaccaggttc acggtcccct cggtcaggcg
     2941 tccgtcgaag tcggtcatgg tcggtctcct ggtgggtggg ggcggggcgc cagcacgaag
     3001 tgccggcgcc ccgcgggggg ttggtcgggt caggcgccga accggcgggc ggcggcggcg
     3061 accaggccgt cggcggcggc catggcgcgg tcgcggtcgg tggtgagggc ggtgcggtcg
     3121 gcggcggccc agtcctgctc tcacgcaacg tctacgtgga cgctcagggc gacacgatcg
     3181 aggtcgggga gtccgtcgct ccggcgggcc gctggcgcgt ccaccgggac tgatcaaggc
     3241 gaatacttca tatgcgggga tcgaccgcgc gggtcccgga cggggaagag cggggagctt
     3301 tgccagagag cgacgacttc cccttgcgtt ggtgattgcc ggtcagggca gccatccgcc
     3361 atcgtcgcgt agggtgtcac accccaggaa tcgcgtcact gaacacagca gccggtagga
     3421 cgaccatgac tgagttggac accatcgcaa atccgtccga tcccgcggtg cagcggatca
     3481 tcgatgtcac caagccgtcg cgatccaaca taaagacaac gttgatcgag gacgtcgagc
     3541 ccctcatgca cagcatcgcg gccggggtgg agttcatcga ggtctacggc agcgacagca
     3601 gtccttttcc atctgagttg ctggatctgt gcgggcggca gaacataccg gtccgcctca
     3661 tcgactcctc gatcgtcaac cagttgttca agggggagcg gaaggccaag acattcggca
     3721 tcgcccgcgt ccctcgcccg gccaggttcg gcgatatcgc gagccggcgt ggggacgtcg
     3781 tcgttctcga cggggtgaag atcgtcggga acatcggcgc gatagtacgc acgtcgctcg
     3841 cgctcggagc gtcggggatc atcctggtcg acagtgacat caccagcatc gcggaccggc
     3901 gtctccaaag ggccagccga ggttacgtct tctcccttcc cgtcgttctc tccggtcgcg
     3961 aggaggccat cgccttcatt cgggacagcg gtatgcagct gatgacgctc aaggcggatg
     4021 gcgacatttc cgtgaaggaa ctcggggaca atccggatcg gctggccttg ctgttcggca
     4081 gcgaaaaggg tgggccttcc gacctgttcg aggaggcgtc ttccgcctcg gtttccatcc
     4141 ccatgatgag ccagaccgag tctctcaacg tttccgtttc cctcggaatc gcgctgcacg
     4201 agaggatcga caggaatctc gcggccaacc gataagcgcc tctgttcctc ggacgctcgg
     4261 ttcctcgacc tcgattcgtc agtgatgatc agacgtcgaa ggtgtagtgc cgggtgtggt
     4321 ccagcagggc cgccggggtg gtgtcgttcc acggcttcat cgtctcgttg aggtcgacca
     4381 aagcggccat cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc
     4441 tgctggtttc ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca
     4501 gcctcaggca gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact
     4561 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     4621 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
     4681 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     4741 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     4801 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     4861 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     4921 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     4981 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     5041 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     5101 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     5161 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     5221 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     5281 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     5341 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     5401 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     5461 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     5521 tcctcatcct gtctcttgat cagatctaaa gttttgtcgt ctttccagac gttagtaaat
     5581 gaattttctg tatgaggttt tgctaaacaa ctttcaacag tttcagcgga gtgagaatag
     5641 aaaggaacaa ctaaaggaat tgcgaataat aattttttca cgttgaaaat ctccaaaaaa
     5701 aaaggctcca aaaggagcct ttatgcggtg tgaaataccg cacagatgcg taaggagaaa
     5761 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     5821 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     5881 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     5941 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     6001 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     6061 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     6121 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     6181 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     6241 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6301 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6361 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattcg
     6421 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ttgttccctt
     6481 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7321 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8641 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8701 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8761 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8821 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     8881 aactatgcgg catcagagca gattgtactg agagtgcacc a
//
