pFGUS2b vector (V006260)

LOCUS       40924_20031       10970 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pFGUS2b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Regulation of NAC2 expression by miR164c mediates fertilizer 
            application-induced delay of leaf senescence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 10970)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10970)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-APR-2013) Section of Molecular Cell "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Suite 8
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     regulatory      complement(930..1310)
                     /note="MAS promoter; mannopine synthase promoter"
                     /regulatory_class="promoter"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      complement(1332..1584)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1593..1695
                     /note="Multiple Cloning Sites (MCS); MCS; synthetic linker 
                     sequence including 9 unique restriction sites"
     intron          1711..1900
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1895..3718
                     /label=GUS
                     /note="beta-glucuronidase"
     terminator      3738..4445
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4474..4490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4693..4717
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6017..6643
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7080..8144
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8213..8407
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8751..8891
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9077..9665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9755..10546)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"