LOCUS       40924_20031       10970 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pFGUS2b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Regulation of NAC2 expression by miR164c mediates fertilizer 
            application-induced delay of leaf senescence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 10970)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10970)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-APR-2013) Section of Molecular Cell "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Suite 8
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     regulatory      complement(930..1310)
                     /note="MAS promoter; mannopine synthase promoter"
                     /regulatory_class="promoter"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     terminator      complement(1332..1584)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1593..1695
                     /note="Multiple Cloning Sites (MCS); MCS; synthetic linker 
                     sequence including 9 unique restriction sites"
     intron          1711..1900
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1895..3718
                     /label=GUS
                     /note="beta-glucuronidase"
     terminator      3738..4445
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(4474..4490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4693..4717
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6017..6643
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             7080..8144
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      8213..8407
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8751..8891
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9077..9665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9755..10546)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tatcgaattc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
     1381 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     1441 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     1501 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     1561 ttattgccaa atgtttgaac gatcgataat tcgttaacga gacgcggacc ggagacgcct
     1621 cagggagacg cacagagtgg agacggacgt cctgacgctc gagaagctta tttaaatcac
     1681 gtgccatggg gatccatggt agatctgagg gtaaatttct agtttttctc cttcattttc
     1741 ttggttagga cccttttctc tttttatttt tttgagcttt gatctttctt taaactgatc
     1801 tattttttaa ttgattggtt atggtgtaaa tattacatag ctttaactga taatctgatt
     1861 actttatttc gtgtgtctat gatgatgatg atagttacag aaccgacgac tcgtccgtcc
     1921 tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga
     1981 tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc
     2041 aattgctgtg ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc
     2101 gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat
     2161 cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt
     2221 gatggagcat cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc
     2281 cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa ctgaactggc agactatccc
     2341 gccgggaatg gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt
     2401 ctttaactat gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt
     2461 ggacgatatc accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg
     2521 gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt
     2581 tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc
     2641 gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat
     2701 ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa
     2761 ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa
     2821 aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa
     2881 ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca
     2941 tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg
     3001 tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac
     3061 tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag
     3121 cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata
     3181 tttcgcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt
     3241 caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg
     3301 cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt
     3361 actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga
     3421 atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga
     3481 gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt
     3541 cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg
     3601 cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct
     3661 gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg
     3721 aactagtccc tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     3781 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     3841 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     3901 taatattctc cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat
     3961 gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata
     4021 acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca
     4081 aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt
     4141 atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg
     4201 ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt
     4261 tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag
     4321 aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga
     4381 cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc
     4441 agcaggacct gcaggcatgc aagctagctt ggcactggcc gtcgttttac aacgtcgtga
     4501 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4561 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4621 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     4681 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     4741 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     4801 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     4861 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     4921 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     4981 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     5041 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     5101 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     5161 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     5221 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     5281 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     5341 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     5401 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     5461 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     5521 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     5581 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     5641 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     5701 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     5761 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     5821 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     5881 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     5941 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     6001 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     6061 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     6121 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     6181 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     6241 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     6301 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     6361 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     6421 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     6481 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     6541 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     6601 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     6661 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     6721 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     6781 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     6841 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     6901 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     6961 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     7021 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     7081 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     7141 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     7201 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     7261 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     7321 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     7381 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     7441 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     7501 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     7561 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     7621 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     7681 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     7741 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     7801 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     7861 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     7921 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     7981 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     8041 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     8101 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     8161 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     8221 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     8281 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     8341 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     8401 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     8461 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     8521 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     8581 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     8641 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     8701 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     8761 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     8821 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     8881 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8941 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9001 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9061 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9121 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9181 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9241 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9301 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9361 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9421 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9481 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     9541 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9601 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9661 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9721 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     9781 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     9841 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     9901 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     9961 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    10021 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    10081 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    10141 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    10201 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    10261 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    10321 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    10381 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    10441 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    10501 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    10561 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    10621 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    10681 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    10741 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    10801 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    10861 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    10921 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
