pFC8K vector (V006288)

LOCUS       V006288                 5039 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006288
VERSION     V006288
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     pFC8K (HaloTag) CMV Flexi Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 5039)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2005) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2005) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 4; type: "Journal Article"
            On Oct 6, 2005 this sequence version replaced DQ137255.1.
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1087..1419
                     /label="barnase"
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     CDS             1456..1467
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1501..2376
                     /gene="dhaA"
                     /label="Haloalkane dehalogenase"
                     /note="Haloalkane dehalogenase from Rhodococcus sp.
                     Accession#: P0A3G3"
     polyA_signal    complement(2513..2634)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             3033..3824
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(3999..4587)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    4703..4986
                     /label="cer region"
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"