LOCUS       V006288                 5039 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006288
VERSION     V006288
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     pFC8K (HaloTag) CMV Flexi Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5039)
  AUTHORS   Hartnett J, Slater M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   4  (bases 1 to 5039)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2005) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2005) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 4; type: "Journal Article"
            On Oct 6, 2005 this sequence version replaced DQ137255.1.
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1087..1419
                     /label="barnase"
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     CDS             1456..1467
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1501..2376
                     /gene="dhaA"
                     /label="Haloalkane dehalogenase"
                     /note="Haloalkane dehalogenase from Rhodococcus sp.
                     Accession#: P0A3G3"
     polyA_signal    complement(2513..2634)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             3033..3824
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(3999..4587)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    4703..4986
                     /label="cer region"
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggaa taagtaagga
     1081 atccacatgg cacaggttat caacacgttt gacggggttg cggattatct tcagacatat
     1141 cataagctac ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca
     1201 tcaaaaggga accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca
     1261 aacagggaag gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac
     1321 tatacatcag gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac
     1381 aaaacaacgg accattatca gacctttaca aaaatcagat aatgtttaat gaccccgtgt
     1441 cgagctctcg atctgatcga aggtcgtggt atccctcgta actctcgtgt tgatgcggga
     1501 tccgaaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
     1561 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
     1621 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
     1681 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
     1741 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
     1801 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
     1861 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
     1921 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
     1981 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
     2041 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
     2101 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
     2161 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
     2221 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
     2281 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
     2341 atcggcagtg agatcgcgcg ctggctcccg gcactctaat ctagagtcga cctgcaggca
     2401 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     2461 ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac
     2521 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2581 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2641 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
     2701 aagtaaaacc tctacaaatg tggtaaaatc gaattctaat ggatcctctt tgcgcttgcg
     2761 ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc
     2821 ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg
     2881 cagcgtgagc ttcaaaagaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct
     2941 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     3001 ctgatcaaga gacaggatga cggtcgtttc gcatgcttga acaagatgga ttgcacgcag
     3061 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3121 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3181 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     3241 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3301 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3361 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     3421 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgcact cggatggaag
     3481 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     3541 tgttcgccag gctcaaggcg cgtatgccgg atggtgagga tctcgtcgtg actcatggcg
     3601 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     3661 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     3721 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     3781 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     3841 gttcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata
     3901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     4441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4561 attacgcgca gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac
     4621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4681 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
     4741 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
     4801 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
     4861 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
     4921 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
     4981 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatct
//
