pERV3 vector (V006528)

LOCUS       V006528                 8363 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006528
VERSION     V006528
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Technical Services, Stratagene, 11011 N.
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 8363)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Road, La Jolla, CA 92037, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(468..600)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          complement(759..824)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(912..2297)
                     /gene="RXRA"
                     /label="Retinoic acid receptor RXR-alpha"
                     /note="Retinoic acid receptor RXR-alpha from Homo sapiens.
                     Accession#: P19793"
     CDS             complement(2295..2324)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    complement(2324..2902)
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2910..4760)
                     /codon_start=1
                     /product="ecdysone receptor VgEcR"
                     /label="ecdysone receptor VgEcR"
                     /protein_id="AAC95155.1"
                     /translation="MLGERKDRASGYHYNALTCGSCKVFFRRSVTKSAVYCCKFGRACE
                     MDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGG
                     NGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIY
                     KLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAF
                     TKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIED
                     LLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHC
                     GDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQS
                     HLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSL
                     TQNDSQHQTQPQLQPQLPPQLQGQLQPQLHPQLQTQLQPQIQPQPQLLPVSAPVPASVT
                     APGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGG
                     NVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA"
     CDS             complement(4844..5077)
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     CDS             complement(5078..5107)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      5107..5116
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5153..5356)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5357..5660)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5855..6443)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(6772..6819)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7054..7845)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7880..8237)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(8239..8343)
                     /label="AmpR promoter"