LOCUS       V006528                 8363 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006528
VERSION     V006528
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Technical Services, Stratagene, 11011 N.
            Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 8363)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (13-OCT-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Road, La Jolla, CA 92037, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(468..600)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     intron          complement(759..824)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(912..2297)
                     /gene="RXRA"
                     /label="Retinoic acid receptor RXR-alpha"
                     /note="Retinoic acid receptor RXR-alpha from Homo sapiens.
                     Accession#: P19793"
     CDS             complement(2295..2324)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    complement(2324..2902)
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2910..4760)
                     /codon_start=1
                     /product="ecdysone receptor VgEcR"
                     /label="ecdysone receptor VgEcR"
                     /protein_id="AAC95155.1"
                     /translation="MLGERKDRASGYHYNALTCGSCKVFFRRSVTKSAVYCCKFGRACE
                     MDMYMRRKCQECRLKKCLAVGMRPECVVPENQCAMKRREKKAQKEKDKMTTSPSSQHGG
                     NGSLASGGGQDFVKKEILDLMTCEPPQHATIPLLPDEILAKCQARNIPSLTYNQLAVIY
                     KLIWYQDGYEQPSEEDLRRIMSQPDENESQTDVSFRHITEITILTVQLIVEFAKGLPAF
                     TKIPQEDQITLLKACSSEVMMLRMARRYDHSSDSIFFANNRSYTRDSYKMAGMADNIED
                     LLHFCRQMFSMKVDNVEYALLTAIVIFSDRPGLEKAQLVEAIQSYYIDTLRIYILNRHC
                     GDSMSLVFYAKLLSILTELRTLGNQNAEMCFSLKLKNRKLPKFLEEIWDVHAIPPSVQS
                     HLQITQEENERLERAERMRASVGGAITAGIDCDSASTSAAAAAAQHQPQPQPQPQPSSL
                     TQNDSQHQTQPQLQPQLPPQLQGQLQPQLHPQLQTQLQPQIQPQPQLLPVSAPVPASVT
                     APGSLSAVSTSSEYMGGSAAIGPITPATTSSITAAVTASSTTSAVPMGNGVGVGVGVGG
                     NVSMYANAQTAMALMGVALHSHQEQLIGGVAVKSEHSTTA"
     CDS             complement(4844..5077)
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     CDS             complement(5078..5107)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     regulatory      5107..5116
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5153..5356)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(5357..5660)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5855..6443)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     polyA_signal    complement(6772..6819)
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7054..7845)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7880..8237)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        complement(8239..8343)
                     /label="AmpR promoter"
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtcc agacatgata
      481 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      541 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      601 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      661 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcatga acagactgtg
      721 aggactgagg ggcctgaaat gagccttggg actgtgatct aaaatacaca aacaattaga
      781 atcagtagtt taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc
      841 tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc
      901 gcggccgcct aagtcatttg gtgcggcgcc tccagcatct ccataaggaa ggtgtcaatg
      961 ggtgtgtccc cgatgagctt gaagaagaag agatgttcca ggcatttgag cccgatggag
     1021 cgcagagccg gcaggcggag caagagctta gcgaaccttc ccggctgctc tgggtacttg
     1081 tgcttgcagt aggcctccaa ggacgcatag accttctccc tcagcgcctc cacctcggcc
     1141 gggttcgaga gccccttgga gtcagggtta aagaggacga tggcgcgcag gcagcccagc
     1201 tccgtcttgt ccatctgcat gtcccgcatc ttggacacaa gctccgtcag caccctgtca
     1261 aagatggcgc ccacccctgc gctgtgggcg ctgttccggt ggacgtgcag cccggtggcc
     1321 aggaggatcc cgtccttcac ggcgatggag cggtgggaga aggaggcgat gagcagctca
     1381 ttccagcctg cccgcagcag gatgacctgg tcgtccaggg gcagctctga gaagtgtggg
     1441 atccgcttgg cccactccac cagggtgaaa agctgtttgt cggctgcttg gcaaatgttg
     1501 gtgacagggt cgttcggcga gctggggttc agccccatgt ttgcctccac gtaggtctcg
     1561 gtcttgggct ccacggccag ctcagcctcc aggatcctct ccaccggcat gtcctcgttg
     1621 gcgctgctgg tcgactccac ctcattctcg ttccggtcct tgccacgctg ccgctcctcc
     1681 tgcacggctt cccgcttcat gcccatggcc aggcacttct ggtagcggca gtactggcac
     1741 cggttccgct gccgcttgtc aatcaggcag tccttgttgt cgcggcaggt gtaggtcagg
     1801 tccttgcgca ccgtccgctt gaagaagccc ttgcacccct cgcagctgta cactccatag
     1861 tgcttgcctg aggagcggtc cccgcagatg gcgcagatgt gcttggtgaa ggaagccatg
     1921 tttcctgagg ggtgggcggg gaccttgagg acgccattga ggcccagggg gggcttgatg
     1981 tcctcgctgc tgctgacggg gttcataggt gagctgagct gggggctgcc agtgctgaag
     2041 cccagggtgg gtgtggtggg caccgacatg gagtgggggc ccatggggga gctgatgacc
     2101 gagaaaggcg ggcccatgcc gttgatgggg gagctcaggg tgctgatggg agaatgcagc
     2161 tgtcccgggg agccgatgcc aggccccagg gacgggtgca gcgagggggc agccatggag
     2221 cctcgccccg tcggggaggt gagggaggag ttcacctggg tggagaaatc gagcggcagg
     2281 aaatgtttgg tgtccaagtc ttcttcagaa ataagttttt gttccatatt atcgatcgtg
     2341 tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt ttaacctcga
     2401 ctaaacacat gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac
     2461 cttctgggca tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc
     2521 cacaactatc caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta
     2581 tacacgtggc ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca
     2641 aagggtcgct acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac
     2701 gacattcaac agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt
     2761 caagaagaca gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga
     2821 aaataacata tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt
     2881 aggggggggg gagggagagg ggggtaaccc tatgcagtcg tcgagtgctc cgacttaacc
     2941 gccactcccc cgataagctg ctcttggtgc gaatgcaggg ctacacccat caaggccatc
     3001 gccgtctggg cgttcgcata catgctgacg ttgccgccca ccccaacacc gactccaact
     3061 ccgttgccca tcggtaccgc tgatgtggtg gagctagcgg taacggcagc cgtgatactg
     3121 ctggtggttg ccggcgtgat gggtcctatg gccgcacttc cgcccatgta ttcgctgctc
     3181 gtactgaccg cggacaagga accaggtgcg gttacggagg cgggcacggg agcggagacg
     3241 ggaaggagct gtggctgtgg ttgaatctgt ggctggagtt gcgtctgaag ctgtgggtgg
     3301 agctggggtt gcagttgacc ttgcagctga ggtggtagct gaggttgtag ctgcggctgt
     3361 gtctggtgct gggaatcgtt ctgggtcagg gaggagggtt ggggctgggg ctgaggctga
     3421 ggctgatgct gggccgcggc tgccgccgcc gaagtggagg cagagtcgca atcaatgccg
     3481 gcggtaatgg cgcccccaac cgatgcccgc atacgctcag cccgctcgag acgctcgttc
     3541 tcctcctggg taatctgaag gtgcgactgg accgatggcg ggatggcatg aacgtcccag
     3601 atctcctcga ggaacttggg cagtttgcgg tttttgagct ttagtgagaa acacatctcg
     3661 gcgttctggt tgcccagcgt acgcagctcg gtgaggatcg agagcagctt tgcgtagaag
     3721 acgaggctca ttgagtcgcc gcagtggcgg ttgagtatat aaatgcgtag cgtgtcgatg
     3781 tagtagctct ggatcgcttc gactagctgg gccttctcca ggcccggccg gtccgagaag
     3841 atcacaatgg cagtgagaag cgcgtattcg acgttgtcca ccttcatcga gaacatttgg
     3901 cggcagaaat gcagcaggtc ttcaatgtta tcagccattc cggccatttt gtaagaatcc
     3961 cgcgtatatg atctattatt cgcgaagaat attgagtccg agctgtggtc atagcgtcgt
     4021 gccatacgca gcatcatcac ctccgacgag caggccttta gtaacgtgat ctggtcctcc
     4081 tggggtatct ttgtaaacgc tggtagacct ttagcaaact caacaatcaa ctggaccgtg
     4141 agtatggtta tctcggttat atgccgaaag ctgacgtccg tttggctctc gttctcatcg
     4201 ggttgactca ttatacgcct gagatcctct tcagatggct gctcatagcc atcctggtac
     4261 caaattaact tgtatataac ggccaactga ttgtacgtta aggaaggtat attgcgcgct
     4321 tgacacttgg ccaatatttc atcaggtagt agcggaatag tggcatgctg gggcggctcg
     4381 catgtcataa ggtcaagaat ctccttctta acaaagtctt ggccgccacc agaggccaag
     4441 ctgccattgc cgccatgctg agagctcggc gaagtggtca ttttgtcctt ctccttctgg
     4501 gccttctttt cgcgccgctt catcgcacat tggttctccg ggacgacgca ttccggccgc
     4561 atacccacgg ccaggcactt tttcaggcgg cactcctgac actttcgcct catgtacatg
     4621 tccatttcgc aggcgcgccc gaacttgcag cagtagacgg cgctcttcgt aacgctgcgt
     4681 cgaaagaaca ccttgcagga tccacaggtg agggcgttgt agtggtagcc ggaggccctg
     4741 tcctttcgtt cgccgagtat ccgttcaagc tgctcgaagg cgagagatca tcgcgacctg
     4801 aagatataga atttgatatt cttctagatg tacctagaag cttcccaccg tactcgtcaa
     4861 ttccaagggc atcggtaaac atctgctcaa actcgaagtc ggccatatcc agagcgccgt
     4921 agggggcgga gtcgtggggg gtaaatcccg gacctgggga atccccgtcc cccaacatgt
     4981 ccagatcgaa atcgtctagc gcgtcggcat gcgccatcgc cacgtcctcg ccgtctaagt
     5041 ggagttcgtc ccccaggctg acatcggtcg ggggggccaa gtcttcttca gaaataagtt
     5101 tttgttccat ggtggcggcc ggccactagc ggatctgacg gttcactaaa ccagctctgc
     5161 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5221 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5281 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5341 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5401 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5461 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5521 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5581 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     5641 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     5701 ttgattacta ttaataacta atgcaacggc gctgcagcca ctgcatggcg gtaatacggt
     5761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     6301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac
     6601 ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg
     6661 aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc
     6721 tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg
     6781 acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
     6841 gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca
     6901 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     6961 acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt
     7021 ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     7081 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     7141 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     7201 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     7261 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc
     7321 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     7381 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     7441 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     7501 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     7561 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     7621 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc
     7681 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     7741 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     7801 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     7861 tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     7921 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     7981 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     8041 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     8101 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     8161 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     8221 ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8281 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8341 gcgcacattt ccccgaaaag tgc
//
