pHA-SP vector (V005618)

LOCUS       40924_24059        3319 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHA-SP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Reverte CG, Yuan L, Keady BT, Lacza C, Attfield KR, Mahon GM, 
            Freeman B, Whitehead IP, Hake LE.
  TITLE     XGef is a CPEB-interacting protein involved in Xenopus oocyte 
            maturation
  JOURNAL   Dev. Biol. 255 (2), 383-398 (2003)
  PUBMED    12648498
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Reverte CG, Hake LE.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2002) Biology, Boston College, 140 Commonwealth 
            Avenue, Chestnut, MA 02467, USA
REFERENCE   3  (bases 1 to 3319)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3319)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev. 
            Biol."; date: "2003"; volume: "255"; issue: "2"; pages: "383-398"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2002) Biology, Boston College, 140 Commonwealth Avenue, 
            Chestnut, MA 02467, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           31..73
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             99..125
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    126..159
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           171..296
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     misc_feature    302..334
                     /label=poly A tail
                     /note="poly A tail"
     misc_feature    352..387
                     /note="contains multiple RNA linearization sites for in
                     vitro transcription"
     primer_bind     complement(406..422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(430..446)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(454..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(499..520)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(808..1396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1570..2427)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2428..2532)
                     /label=AmpR promoter