LOCUS       40924_24059        3319 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHA-SP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Reverte CG, Yuan L, Keady BT, Lacza C, Attfield KR, Mahon GM, 
            Freeman B, Whitehead IP, Hake LE.
  TITLE     XGef is a CPEB-interacting protein involved in Xenopus oocyte 
            maturation
  JOURNAL   Dev. Biol. 255 (2), 383-398 (2003)
  PUBMED    12648498
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Reverte CG, Hake LE.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2002) Biology, Boston College, 140 Commonwealth 
            Avenue, Chestnut, MA 02467, USA
REFERENCE   3  (bases 1 to 3319)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3319)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev. 
            Biol."; date: "2003"; volume: "255"; issue: "2"; pages: "383-398"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2002) Biology, Boston College, 140 Commonwealth Avenue, 
            Chestnut, MA 02467, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     5'UTR           31..73
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             99..125
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    126..159
                     /label=multiple cloning site
                     /note="multiple cloning site"
     3'UTR           171..296
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     misc_feature    302..334
                     /label=poly A tail
                     /note="poly A tail"
     misc_feature    352..387
                     /note="contains multiple RNA linearization sites for in
                     vitro transcription"
     primer_bind     complement(406..422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(430..446)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(454..484)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(499..520)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(808..1396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1570..2427)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2428..2532)
                     /label=AmpR promoter
ORIGIN
        1 atttaggtga cactatagaa tacaagcttg cttgttcttt ttgcagaagc tcagaataaa
       61 cgctcaactt tggcagatcg gtaccgaatt caatggaata cccttacgat gtacccgatt
      121 atgcccatat ggccatggta ccgcggccgc tcgagatctg gttaccacta aaccagcctc
      181 aagaacaccc gaatggagtc tctaagctac ataataccaa cttacacttt acaaaatgtt
      241 gtcccccaaa atgtagccat tcgtatctgc tcctaataaa aagaaagttt cttcacattc
      301 taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaacccccc cccccccccc cctgcaggtc
      361 gactctagag gatccccggg cgagctcgaa ttaattcgta atcatgtcat agctgtttcc
      421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      541 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      841 caaaaatcga cgctcaagtc aagaggtggc gaaacccgac aggactataa agatccaggc
      901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2461 tcagggttat tgtctcatga gcggatatat atttgaatgt atttagaaaa ataaacaaat
     2521 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2581 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2641 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2701 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2761 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc attcgacgct
     2821 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     2881 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     2941 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     3001 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     3061 tgccggccac gatgcgtccg gcgtagagga tctggctagc gatgaccctg ctgattggtt
     3121 cgctgaccat ttccgggtgc gggacggcgt taccagaaac tcagaaggtt cgtccaacca
     3181 aaccgactct gacggcagtt tacgagagag atgatagggt ctgcttcagt aagccagatg
     3241 ctacacaatt aggcttgtac atattgtcgt tagaacgcgg ctacaattaa tacataacct
     3301 tatgtatcat acacatacg
//
