pGreenII-35S vector (V005890)

LOCUS       40924_22607        3255 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pGreenII-35S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Tuo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2016) Papaya Biotechnology Laboratory, Institute 
            of Tropical Bioscience and Biotechnology, Chinese Academy of 
            Tropical Agricultural Science, 4 Xueyuan Road, Haikou, Hainan, 
            Haikou, Hannan Province 571101, China
REFERENCE   2  (bases 1 to 3255)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-SEP-2016) Papaya Biotechnology Laboratory, Institute of Tropical
            Bioscience and Biotechnology, Chinese Academy of Tropical 
            Agricultural Science, 4 Xueyuan Road, Haikou, Hainan, Haikou, Hannan
            Province 571101, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(572..748)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    797..843
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(841..1186)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1247..1271
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1362..1950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2124..2936)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3227..3255
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"