LOCUS       40924_22607        3255 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pGreenII-35S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Tuo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2016) Papaya Biotechnology Laboratory, Institute 
            of Tropical Bioscience and Biotechnology, Chinese Academy of 
            Tropical Agricultural Science, 4 Xueyuan Road, Haikou, Hainan, 
            Haikou, Hannan Province 571101, China
REFERENCE   2  (bases 1 to 3255)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-SEP-2016) Papaya Biotechnology Laboratory, Institute of Tropical
            Bioscience and Biotechnology, Chinese Academy of Tropical 
            Agricultural Science, 4 Xueyuan Road, Haikou, Hainan, Haikou, Hannan
            Province 571101, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(572..748)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    797..843
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(841..1186)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1247..1271
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1362..1950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2124..2936)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3227..3255
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cgatctggat tttagtactg gattttggtt
      601 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
      661 ttcttatatg ctcaacacat gagcgaaacc ctataagaac cctaatttcc cttatcggga
      721 aactactcac acattattta tggagaaaat agagagagat agatttgtag agagagactg
      781 gtgatttcag cgtaccgaat tcgagctcgc ccggggatcc tctagagtcg aagcttgggc
      841 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
      901 ggattgtgcg tcatccctta cgtcagtgga gatgtcacat caatccactt gctttgaaga
      961 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     1021 accactgtcg gcagaggcat cttgaatgat agcctttcct ttatcgcaat gatggcattt
     1081 gtaggagcca ccttcctttt ctactgtcct ttcgatgaag tgacagatag ctgggcaatg
     1141 gaatccgagg aggtttcccg aaattaccct ttgttgaaaa gtctcaatag ccctttggtc
     1201 ttctgagact gtatctttga catttttgga gtaggggtac gatccttgac aggatatatt
     1261 ggcgggtaaa ctaagtcgct gtatgtgttt gtttgagatc tcatgtgagc aaaaggccag
     1321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaag aagagttggt
     1861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2101 gtgtaacatt ggtctagtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     2161 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2221 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     2281 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     2341 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     2401 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     2461 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     2521 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2581 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca
     2641 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2701 ataaattccg tcagccagtt tagtctgacc atctcatctg taacaacatt ggcaacgcta
     2761 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt
     2821 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     2881 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca
     2941 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
     3001 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
     3061 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
     3121 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
     3181 ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat ccaacagccc
     3241 gccgtcgagc gggct
//
