pGR8 vector (V005898)

LOCUS       40924_22543        9655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGR8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Mazzarella R, Pilia G.
  TITLE     Recombination trapping: an 'in vivo' approach to recover cDNAs 
            encoded in YACs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9655)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     GSDB:S:1274444.
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..135
                     /standard_name="telomere"
                     /label=telomere
     prim_transcript 145..2097
                     /gene="HIS5"
     gene            145..2097
                     /gene="HIS5"
                     /label=HIS5
     CDS             761..1915
                     /codon_start=1
                     /gene="HIS5"
                     /product="histidinol phosphate aminotransferase"
                     /EC_number="2.6.1.9"
                     /label=HIS5
                     /protein_id="AAB68950.1"
                     /translation="MVFDLKRIVRPKIYNLEPYRCARDDFTEGILLDANENAHGPTPVE
                     LSKTNLHRYPDPHQLEFKTAMTKYRNKTSSYANDPEVKPLTADNLCLGVGSDESIDAII
                     RACCVREEKILVLPPTYSMSSVCANINDIEVVQCPLIVSDGSFQMDTEAVLTILKNDSL
                     IKLMFVTSPGNPTGAKIKTSLIEKVLQNWDNGLVVVDEAYVDFCGGSTAPLVTKYPNLV
                     TLQTLSKSFGLAGIRLGMTYATAELARILNAMKAPYNISSLASEYALKAVQDSNLKKME
                     ATSKIINEEKMLLLKELTALDYVDDQYVGGLDANFLLIRINGGDNVLAKKLYYQLATQS
                     GVVVRFRGNELGCSGCLRITVGTHEENTHLIKYFKETLYKLANE"
     promoter        complement(2100..2118)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2136..2373
                     /label=LYS2 promoter
     CDS             2374..6549
                     /label=LYS2
                     /note="alpha-aminoadipate reductase, required for lysine 
                     biosynthesis"
     rep_origin      complement(6719..7147)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7289..7305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7312..7330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7339..7446
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(7459..7477)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7498..7514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7522..7538)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7546..7576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7591..7612)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7900..8488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8662..9519)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9520..9624)
                     /label=AmpR promoter