LOCUS       40924_22543        9655 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGR8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Mazzarella R, Pilia G.
  TITLE     Recombination trapping: an 'in vivo' approach to recover cDNAs 
            encoded in YACs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9655)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     GSDB:S:1274444.
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..135
                     /standard_name="telomere"
                     /label=telomere
     prim_transcript 145..2097
                     /gene="HIS5"
     gene            145..2097
                     /gene="HIS5"
                     /label=HIS5
     CDS             761..1915
                     /codon_start=1
                     /gene="HIS5"
                     /product="histidinol phosphate aminotransferase"
                     /EC_number="2.6.1.9"
                     /label=HIS5
                     /protein_id="AAB68950.1"
                     /translation="MVFDLKRIVRPKIYNLEPYRCARDDFTEGILLDANENAHGPTPVE
                     LSKTNLHRYPDPHQLEFKTAMTKYRNKTSSYANDPEVKPLTADNLCLGVGSDESIDAII
                     RACCVREEKILVLPPTYSMSSVCANINDIEVVQCPLIVSDGSFQMDTEAVLTILKNDSL
                     IKLMFVTSPGNPTGAKIKTSLIEKVLQNWDNGLVVVDEAYVDFCGGSTAPLVTKYPNLV
                     TLQTLSKSFGLAGIRLGMTYATAELARILNAMKAPYNISSLASEYALKAVQDSNLKKME
                     ATSKIINEEKMLLLKELTALDYVDDQYVGGLDANFLLIRINGGDNVLAKKLYYQLATQS
                     GVVVRFRGNELGCSGCLRITVGTHEENTHLIKYFKETLYKLANE"
     promoter        complement(2100..2118)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2136..2373
                     /label=LYS2 promoter
     CDS             2374..6549
                     /label=LYS2
                     /note="alpha-aminoadipate reductase, required for lysine 
                     biosynthesis"
     rep_origin      complement(6719..7147)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7289..7305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7312..7330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7339..7446
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(7459..7477)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7498..7514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7522..7538)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7546..7576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7591..7612)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7900..8488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8662..9519)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9520..9624)
                     /label=AmpR promoter
ORIGIN
        1 gcatgcctgc aggtcgacgc ccccctgcca ccacacccac accacaccca caccacaccc
       61 acacccacac acccacacac ccacacaccc acacccacac ccacacactc tctcacatct
      121 acctctactc tcggggcatc gataagcttt agctggcaat tcttgtactg tctttcgccg
      181 aaacctgttt gatgactact tcatcaattt tttttttttc tgccgcattc caaaggtcat
      241 aactttgcaa aaataaaggg taaatggtta aaaattgtta tcataaataa ggtgaccggt
      301 tatattgaga cctttcctgg acggtaacta atacagaagc cattggtaat gcaataattt
      361 atttgatcat gtgaccacga tccgggtgag actattcaaa aaaggagtca agcattgaaa
      421 taattaatga ctaatccgaa gttaattgtt aggagtcaat tgttttttcc aatgaatgga
      481 atctgagatg actaaactac caattttcaa tagttcatgg tatagtgacg tattagtgct
      541 tttttttctt ggatctgttg actcacttca attgatgttt cttaccctga catgacatac
      601 ttgatatttt atctctcacg ttatataact tgaaaaggat gcacacagtt ctgttcaata
      661 taccctccaa tatgtaaaaa cagtttttcc attgattacg cttaatttgt ttcctgctaa
      721 accagcagta cgtgtgtgtc gtatatatta aaattacact atggtttttg atttgaaaag
      781 aattgttaga ccaaaaattt ataacttgga accttatcgc tgtgcaagag atgatttcac
      841 cgagggtata ttgctagacg ccaatgaaaa tgcccatggt cctactccag ttgaattgag
      901 caagaccaat ttacatcgtt acccagatcc tcaccaattg gaattcaaga ccgcaatgac
      961 gaaatacagg aacaaaacaa gcagttatgc caatgaccca gaggtaaaac ctttaactgc
     1021 tgacaatctg tgcctaggtg tgggatctga tgagagtatt gatgctatta ttagagcatg
     1081 ctgtgtccgg gaagaaaaga ttctggttct tccaccaaca tattctatgt cttctgtttg
     1141 tgcaaatatt aatgatatag aagtcgtcca atgtccttta attgtttccg acggttcttt
     1201 tcaaatggat accgaagctg tattaaccat tttgaaaaac gactcgctaa ttaagttgat
     1261 gttcgttact tcaccaggta atccaaccgg agccaaaatt aagaccagtt taatcgaaaa
     1321 ggtcttacag aattgggaca atgggttagt cgttgttgat gaagcttacg tagatttttg
     1381 tggtggctct acagctccac tagtcaccaa gtatcctaac ttggttactt tgcaaactct
     1441 atccaagtca ttcggtttag ccgggattag gttgggtatg acatatgcaa cagcagagtt
     1501 ggccagaatt ttaaatgcaa tgaaggcgcc ttataatatt tcctccctag cctctgaata
     1561 tgcactaaaa gctgttcaag acagtaatct aaagaagatg gaagccactt cgaaaataat
     1621 caatgaagag aaaatgctcc tcttaaagga attaactgct ttggattacg ttgatgacca
     1681 atatgttggt ggattagatg ctaattttct tttaatacgg atcaacgggg gtgacaatgt
     1741 cttggcaaag aagttatatt accaattggc tactcaatct ggggttgtcg tcagatttag
     1801 aggtaacgaa ttaggctgtt ccggatgttt gagaattacc gttggaaccc atgaggagaa
     1861 cacacatttg ataaagtact tcaaggagac gttatataag ctggccaatg aataaataga
     1921 ttaatttaaa cagtatatgt acagttttat atatatatat acatatataa agaaacctgt
     1981 gcgttttttg tattttcaaa tacatttagt tttggctcct cttctataaa aggctcatgt
     2041 atattgttac agtttttctt cgtttctcat gccctgtttc tatgatagag gatgcatatt
     2101 ctatagtgtc acctaaatct gcggccccat cgatgatcca cttgcaatta cataaaaaat
     2161 tccggcggtt tttcgcgtgt gactcaatgt cgaaatacct gcctaatgaa catgaacatc
     2221 gcccaaatgt atttgaagac ccgctgggag aagttcaaga tatataagta acaagcagcc
     2281 aatagtataa aaaaaaatct gagtttatta cctttcctgg aatttcagtg aaaaactgct
     2341 aattatagag agatatcaca gagttactca ctaatgacta acgaaaaggt ctggatagag
     2401 aagttggata atccaactct ttcagtgtta ccacatgact ttttacgccc acaacaagaa
     2461 ccttatacga aacaagctac atattcgtta cagctacctc agctcgatgt gcctcatgat
     2521 agtttttcta acaaatacgc tgtcgctttg agtgtatggg ctgcattgat atatagagta
     2581 accggtgacg atgatattgt tctttatatt gcgaataaca aaatcttaag attcaatatt
     2641 caaccaacgt ggtcatttaa tgagctgtat tctacaatta acaatgagtt gaacaagctc
     2701 aattctattg aggccaattt ttcctttgac gagctagctg aaaaaattca aagttgccaa
     2761 gatctggaaa ggacccctca gttgttccgt ttggcctttt tggaaaacca agatttcaaa
     2821 ttagacgagt tcaagcatca tttagtggac tttgctttga atttggatac cagtaataat
     2881 gcgcatgttt tgaacttaat ttataacagc ttactgtatt cgaatgaaag agtaaccatt
     2941 gttgcggacc aatttactca atatttgact gctgcgctaa gcgatccatc caattgcata
     3001 actaaaatct ctctgatcac cgcatcatcc aaggatagtt tacctgatcc aactaagaac
     3061 ttgggctggt gcgatttcgt ggggtgtatt cacgacattt tccaggacaa tgctgaagcc
     3121 ttcccagaga gaacctgtgt tgtggagact ccaacactaa attccgacaa gtcccgttct
     3181 ttcacttatc gcgacatcaa ccgcacttct aacatagttg cccattattt gattaaaaca
     3241 ggtatcaaaa gaggtgatgt agtgatgatc tattcttcta ggggtgtgga tttgatggta
     3301 tgtgtgatgg gtgtcttgaa agccggcgca accttttcag ttatcgaccc tgcatatccc
     3361 ccagccagac aaaccattta cttaggtgtt gctaaaccac gtgggttgat tgttattaga
     3421 gctgctggac aattggatca actagtagaa gattacatca atgatgaatt ggagattgtt
     3481 tcaagaatca attccatcgc tattcaagaa aatggtacca ttgaaggtgg caaattggac
     3541 aatggcgagg atgttttggc tccatatgat cactacaaag acaccagaac aggtgttgta
     3601 gttggaccag attccaaccc aaccctatct ttcacatctg gttccgaagg tattcctaag
     3661 ggtgttcttg gtagacattt ttccttggct tattatttca attggatgtc caaaaggttc
     3721 aacttaacag aaaatgataa attcacaatg ctgagcggta ttgcacatga tccaattcaa
     3781 agagatatgt ttacaccatt atttttaggt gcccaattgt atgtccctac tcaagatgat
     3841 attggtacac cgggccgttt agcggaatgg atgagtaagt atggttgcac agttacccat
     3901 ttaacacctg ccatgggtca attacttact gcccaagcta ctacaccatt ccctaagtta
     3961 catcatgcgt tctttgtggg tgacatttta acaaaacgtg attgtctgag gttacaaacc
     4021 ttggcagaaa attgccgtat tgttaatatg tacggtacca ctgaaacaca gcgtgcagtt
     4081 tcttatttcg aagttaaatc aaaaaatgac gatccaaact ttttgaaaaa attgaaagat
     4141 gtcatgcctg ctggtaaagg tatgttgaac gttcagctac tagttgttaa caggaacgat
     4201 cgtactcaaa tatgtggtat tggcgaaata ggtgagattt atgttcgtgc aggtggtttg
     4261 gccgaaggtt atagaggatt accagaattg aataaagaaa aatttgtgaa caactggttt
     4321 gttgaaaaag atcactggaa ttatttggat aaggataatg gtgaaccttg gagacaattc
     4381 tggttaggtc caagagatag attgtacaga acgggtgatt taggtcgtta tctaccaaac
     4441 ggtgactgtg aatgttgcgg tagggctgat gatcaagtta aaattcgtgg gttcagaatc
     4501 gaattaggag aaatagatac gcacatttcc caacatccat tggtaagaga aaacattact
     4561 ttagttcgca aaaatgccga caatgagcca acattgatca catttatggt cccaagattt
     4621 gacaagccag atgacttgtc taagttccaa agtgatgttc caaaggaggt tgaaactgac
     4681 cctatagtta agggcttaat cggttaccat cttttatcca aggacatcag gactttctta
     4741 aagaaaagat tggctagcta tgctatgcct tccttgattg tggttatgga taaactacca
     4801 ttgaatccaa atggtaaagt tgataagcct aaacttcaat tcccaactcc caagcaatta
     4861 aatttggtag ctgaaaatac agtttctgaa actgacgact ctcagtttac caatgttgag
     4921 cgcgaggtta gagacttatg gttaagtata ttacctacca agccagcatc tgtatcacca
     4981 gatgattcgt ttttcgattt aggtggtcat tctatcttgg ctaccaaaat gatttttacc
     5041 ttaaagaaaa agctgcaagt tgatttacca ttgggcacaa ttttcaagta tccaacgata
     5101 aaggcctttg ccgcggaaat tgacagaatt aaatcatcgg gtggatcatc tcaaggtgag
     5161 gtcgtcgaaa atgtcactgc aaattatgcg gaagacgcca agaaattggt tgagacgcta
     5221 ccaagttcgt acccctctcg agaatatttt gttgaaccta atagtgccga aggaaaaaca
     5281 acaattaatg tgtttgttac cggtgtcaca ggatttctgg gctcctacat ccttgcagat
     5341 ttgttaggac gttctccaaa gaactacagt ttcaaagtgt ttgcccacgt cagggccaag
     5401 gatgaagaag ctgcatttgc aagattacaa aaggcaggta tcacctatgg tacttggaac
     5461 gaaaaatttg cctcaaatat taaagttgta ttaggcgatt tatctaaaag ccaatttggt
     5521 ctttcagatg agaagtggat ggatttggca aacacagttg atataattat ccataatggt
     5581 gcgttagttc actgggttta tccatatgcc aaattgaggg atccaaatgt tatttcaact
     5641 atcaatgtta tgagcttagc cgccgtcggc aagccaaagt tctttgactt tgtttcctcc
     5701 acttctactc ttgacactga atactacttt aatttgtcag ataaacttgt tagcgaaggg
     5761 aagccaggca ttttagaatc agacgattta atgaactctg caagcgggct cactggtgga
     5821 tatggtcagt ccaaatgggc tgctgagtac atcattagac gtgcaggtga aaggggccta
     5881 cgtgggtgta ttgtcagacc aggttacgta acaggtgcct ctgccaatgg ttcttcaaac
     5941 acagatgatt tcttattgag atttttgaaa ggttcagtcc aattaggtaa gattccagat
     6001 atcgaaaatt ccgtgaatat ggttccagta gatcatgttg ctcgtgttgt tgttgctacg
     6061 tctttgaatc ctcccaaaga aaatgaattg gccgttgctc aagtaacggg tcacccaaga
     6121 atattattca aagactactt gtatacttta cacgattatg gttacgatgt cgaaatcgaa
     6181 agctattcta aatggaagaa atcattggag gcgtctgtta ttgacaggaa tgaagaaaat
     6241 gcgttgtatc ctttgctaca catggtctta gacaacttac ctgaaagtac caaagctccg
     6301 gaactagacg ataggaacgc cgtggcatct ttaaagaaag acaccgcatg gacaggtgtt
     6361 gattggtcta atggaatagg tgttactcca gaagaggttg gtatatatat tgcattttta
     6421 aacaaggttg gatttttacc tccaccaact cataatgaca aacttccact gccaagtata
     6481 gaactaactc aagcgcaaat aagtctagtt gcttcaggtg ctggtgctcg tggaagctcc
     6541 gcagcagctt aaggttgagc attacgtatg atatgtccat gtacaataat taaatatgaa
     6601 ttaggagaaa gacttagctt cttttcgggt gatgtcactt aaaaactccg agaataatat
     6661 ataataagag aataaaatat tagttattga ataagaactg taaatcagat tttgttaaaa
     6721 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     6781 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     6841 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6901 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6961 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     7021 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     7081 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     7141 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7201 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7261 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     7321 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc
     7381 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc
     7441 ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     7501 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7561 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7621 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7681 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7741 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7801 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7861 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7921 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7981 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8041 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8101 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8161 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8221 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8281 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8341 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8401 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8461 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8521 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8581 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8641 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8701 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8761 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8821 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8881 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8941 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9001 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9061 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9121 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9181 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9241 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9301 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9361 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9421 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9481 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9541 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9601 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
