pGP-Tn7-Tp vector (V005914)

LOCUS       40924_22473        6229 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mini-Tn7 cloning and delivery vector pGP-Tn7-Tp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in 
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
  PUBMED    22706059
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Microbiology and Biothechnology, 
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval, 
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17"; 
            pages: "6001-6008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2012) Microbiology and Biothechnology, INRS-Institut 
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   189..387
                     /note="Tn7R; right end of Tn7"
     primer_bind     392..408
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      436..530
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      633..719
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    765..812
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        897..987
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    1017..1064
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1018..1064
                     /note="FRT; Flp recombinase target site"
     CDS             complement(1129..1362)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(1688..1716)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1768..1815
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1768..1814
                     /note="FRT; Flp recombinase target site"
     protein_bind    1905..1952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1905..1951
                     /note="FRT; Flp recombinase target site"
     misc_feature    1979..2064
                     /note="MCS; multiple cloning site"
     mobile_element  complement(2065..2230)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        2626..2730
                     /label=AmpR promoter
     CDS             2731..3588
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(4660..5028)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5061..5170)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5814..6170
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"