LOCUS       40924_22473        6229 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mini-Tn7 cloning and delivery vector pGP-Tn7-Tp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Chromosomal complementation using Tn7 transposon vectors in 
            Enterobacteriaceae
  JOURNAL   Appl. Environ. Microbiol. 78 (17), 6001-6008 (2012)
  PUBMED    22706059
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Crepin S, Harel J, Dozois CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2012) Microbiology and Biothechnology, 
            INRS-Institut Armand-Frappier, 531, boul. des Prairies, Laval, 
            Quebec H7V 1B7, Canada
REFERENCE   3  (bases 1 to 6229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "17"; 
            pages: "6001-6008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2012) Microbiology and Biothechnology, INRS-Institut 
            Armand-Frappier, 531, boul. des Prairies, Laval, Quebec H7V 1B7, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   189..387
                     /note="Tn7R; right end of Tn7"
     primer_bind     392..408
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      436..530
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      633..719
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    765..812
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        897..987
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    1017..1064
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1018..1064
                     /note="FRT; Flp recombinase target site"
     CDS             complement(1129..1362)
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(1688..1716)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1768..1815
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1768..1814
                     /note="FRT; Flp recombinase target site"
     protein_bind    1905..1952
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_recomb     1905..1951
                     /note="FRT; Flp recombinase target site"
     misc_feature    1979..2064
                     /note="MCS; multiple cloning site"
     mobile_element  complement(2065..2230)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        2626..2730
                     /label=AmpR promoter
     CDS             2731..3588
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(4660..5028)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(5061..5170)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      5814..6170
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 aattgtaccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
       61 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctaga
      121 ggaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
      181 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
      241 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
      301 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
      361 tgcccgtcgt attaaagagg ggcgtggggt tcgaggtcga cggtatcgat aagctagctt
      421 aattagctga gcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
      481 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaagctaga
      541 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
      601 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      721 cgccgctagg agcttgcggc ccggacgatc gaattgggga tcttgaagta cctattccga
      781 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagctg atgtgcttaa
      841 aaacttactc aatggaataa ttctagatgg gccaactttt ggcgaaaatg agacgttgat
      901 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      961 tttgagttat cgagattttc aggagctaag ggtaccgagc tcgaattggg gatcttgaag
     1021 tacctattcc gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc
     1081 tgatgtgctt aaaaacttac tcaatggaat aattctagat aattcttagg ccacacgttc
     1141 aagtgcagcc acaggataaa tttgcactga gcctgggtgg gattcggact cgaccgcata
     1201 gccttcagga gtgagttttg tgcaatacca accgacgact tgaccctgcc aagcggcacc
     1261 agatttcttg cgtacgcgat cccctaagcc aaaggtggca ctcaggggaa gcgcaaactg
     1321 ccctgcaacg ggagcgttgg cttcatcgct actttgaccc atgtcgaatc cttcttgtga
     1381 atctattatg gcgacaagca aatcgagctc tgactgccta ccccacaaca actatcagaa
     1441 agcaccagca caacggctgc ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     1501 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1561 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     1621 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     1681 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgaat tatccattgc
     1741 tgttgacaaa gggaatcagg ggatcttgaa gttcctattc cgaagttcct attctctaga
     1801 aagtatagga acttcagagc gcttttgaag ctaattcgag ctcggtaccc cgaaagcaaa
     1861 ttcgacccat ccattgctgt tgacaaaggg aatcagggga tcttgaagtt cctattccga
     1921 agttcctatt ctctagaaag tataggaact tcagagcgct tttgaagcta attcgatcat
     1981 gcatgagctc actagtggat cccccgggct gcaggaattc ctcgagaagc ttgggcccgg
     2041 tacctcgcga aggccttgca ggccaaccag ataagtgaaa tctagttcca aactattttg
     2101 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     2161 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     2221 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg
     2281 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2341 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2581 aactcgacct gcagatctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2641 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2701 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2761 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2821 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2881 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2941 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     3001 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3061 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3121 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3181 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3241 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     3301 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3361 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3421 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3481 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3541 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3601 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt ttggtgcact
     3661 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     3721 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3781 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3841 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcaaggagat ggcgcccaac
     3901 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     3961 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     4021 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca gccgaccagg
     4081 ctttccacgc ccgcgtgccg ctccatgtcg ttcgcgcggt tctcggaaac gcgctgccgc
     4141 gtttcgtgat tgtcacgctc aagcccgtag tcccgttcga gcgtcgcgca gaggtcagcg
     4201 agggcgcggt aggcccgata cggctcatgg atggtgtttc gggtcgggtg aatcttgttg
     4261 atggcgatat ggatgtgcag gttgtcggtg tcgtgatgca cggcactgac gcgctgatgc
     4321 tcggcgaagc caagcccagc gcagatgcgg tcctcaatcg cgcgcaacgt ctccgcgtcg
     4381 ggcttctctc ccgcgcggaa gctaaccagc aggtgatagg tcttgtcggc ctcggaacgg
     4441 gtgttgccgt gctgggtcgc catcacctcg gccatgacag cgggcagggt gtttgcctcg
     4501 cagttcgtga cgcgcacgtg acccaggcgc tcggtcttgc cttgctcgtc ggtgatgtac
     4561 ttcaccagct ccgcgaagtc gctcttcttg atggagcgca tggggacgtg cttggcaatc
     4621 acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat ggctctgccc tcgggcggac
     4681 cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc
     4741 gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag
     4801 caggccgccc aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata
     4861 gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg ccgacaggct
     4921 catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac
     4981 cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc
     5041 atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
     5101 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
     5161 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
     5221 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
     5281 gaagcagggt tatgcagcgg aaaagcgctg cttccctgct gttttgtgga atatctaccg
     5341 actggaaaca ggcaaatgca ggaaattact gaactgaggg gacaggcgag agacgatgcc
     5401 aaagagctac accgacgagc tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg
     5461 gcgtgatgag gctgcggttg cgttcctggc ggtgagggcg gatgtcgagg cggcgttagc
     5521 gtccggctat gcgctcgtca ccatttggga gcacatgcgg gaaacgggga aggtcaagtt
     5581 ctcctacgag acgttccgct cgcacgccag gcggcacatc aaggccaagc ccgccgatgt
     5641 gcccgcaccg caggccaagg ctgcggaacc cgcgccggca cccaagacgc cggagccacg
     5701 gcggccgaag caggggggca aggctgaaaa gccggccccc gctgcggccc cgaccggctt
     5761 caccttcaac ccaacaccgg acaaaaagga tccagcagtt caacctgttg atagtacgta
     5821 ctaagctctc atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt
     5881 aacgaactaa accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc
     5941 atgtttcacg tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta
     6001 aatagcctct aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc
     6061 ttttaaggtt taacggttgt ggacaacaag ccagggatgt aacgcactga gaagccctta
     6121 gagcctctca aagcaatttt cagtgacaca ggaacactta acggctgaca tggccttaga
     6181 aggcgtttta gaagccctaa tggatggagt caaaacaaac tagcgatcg
//
