pGL4.23-Lmi-PCre vector (V005947)

LOCUS       V005947                 4363 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005947
VERSION     V005947
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Koksharov M.
  TITLE     Design of short-lived firefly luciferase transcriptional reporters
            in mammalian cells: effects of N- or C-terminal modifications and
            interspecies differences on reporter half-life
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Koksharov M, Ugarova N.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutant beetle
            luciferases: novel mutants illuminate luciferase color determinants
            and factors of protein stability in living cells
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4363)
  AUTHORS   Koksharov M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   4  (bases 1 to 4363)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..144
                     /note="multiple cloning region with an inserted
                     CRE-dependent promoter"
     regulatory      27..125
                     /note="CREB-dependent promoter PCre (4x CREB binding
                     sites)"
                     /regulatory_class="promoter"
     protein_bind    44..51
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB palindromic site"
     protein_bind    70..74
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB half-site"
     protein_bind    80..86
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB non-palindromic site"
     protein_bind    102..109
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB palindromic site"
     promoter        151..182
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             215..1852
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     polyA_signal    complement(1917..2038)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2198..2216)
                     /label="RV4 reverse primer binding region"
                     /note="RV4 reverse primer binding region"
     rep_origin      complement(2457..3045)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3248..4105)
                     /label="AmpR"
                     /note="beta-lactamase"
     polyA_signal    4210..4258
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4272..4363
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"