LOCUS       V005947                 4363 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005947
VERSION     V005947
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Koksharov M.
  TITLE     Design of short-lived firefly luciferase transcriptional reporters
            in mammalian cells: effects of N- or C-terminal modifications and
            interspecies differences on reporter half-life
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Koksharov M, Ugarova N.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutant beetle
            luciferases: novel mutants illuminate luciferase color determinants
            and factors of protein stability in living cells
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4363)
  AUTHORS   Koksharov M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   4  (bases 1 to 4363)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (17-OCT-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..144
                     /note="multiple cloning region with an inserted
                     CRE-dependent promoter"
     regulatory      27..125
                     /note="CREB-dependent promoter PCre (4x CREB binding
                     sites)"
                     /regulatory_class="promoter"
     protein_bind    44..51
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB palindromic site"
     protein_bind    70..74
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB half-site"
     protein_bind    80..86
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB non-palindromic site"
     protein_bind    102..109
                     /label="CREB binding site"
                     /bound_moiety="CREB"
                     /note="CREB palindromic site"
     promoter        151..182
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             215..1852
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     polyA_signal    complement(1917..2038)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2198..2216)
                     /label="RV4 reverse primer binding region"
                     /note="RV4 reverse primer binding region"
     rep_origin      complement(2457..3045)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3248..4105)
                     /label="AmpR"
                     /note="beta-lactamase"
     polyA_signal    4210..4258
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4272..4363
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcgcaccaga cagtgacgtc agctgccaga
       61 tcccatggcc gtcatactgt gacgtctttc agacacccca ttgacgtcaa tgggagaaca
      121 gatctggcct cggcggccaa gcttagacac tagagggtat ataatggaag ctcgacttcc
      181 agcttggcaa tccggtactg ttggtaaagc caccatggaa atggaaaagg aggagaatgt
      241 tgtatatggc cctctgccat tctaccccat tgaagaagga tcagctggaa ttcagttgca
      301 taagtacatg catcaatatg ccaaacttgg agcaattgct ttttcgaacg cccttactgg
      361 agttgacatt tcttaccaag aatactttga tattacatgt cgtttagctg aggccatgaa
      421 aaactttggt atgaaaccgg aagaacatat tgctttgtgc agtgaaaatt gtgaagaatt
      481 tttcatccct gtacttgctg gtctttacat tggggtagct gttgcaccta ctaatgaaat
      541 ttacacattg cgtgaactta atcatagttt gggcatcgca caaccaacta ttgtattcag
      601 ctcgagaaaa ggcttaccta aagttttaga agtgcaaaaa acagttacat gcatcaaaaa
      661 aattgttatt ttagatagta aagtaaactt tgggggccac gattgtatgg aaacttttat
      721 taagaaacat gtagaattag gttttcaacc aagtagcttt gtacccattg atgtaaagaa
      781 ccgtaaacaa cacgttgctt tgcttatgaa ttcttctggc tctactggtt tacctaaagg
      841 tgtacgaatt acccacgaag gtgcagttac aagattctca cacgctaagg atccaattta
      901 cggaaaccaa gtttcacctg gtactgctat tttaactgtc gttccgttcc atcatggatt
      961 tggaatgttt accactttag gatactttgc ttgtggatac cgtgttgtaa tgttaacaaa
     1021 atttgatgaa gaactatttt tgagaacttt gcaagattat aagtgtacca gtgttattct
     1081 ggtaccaacg ttatttgcta ttctcaacaa gagtgaattg atcgataagt tcgatttatc
     1141 taatctaact gaaattgctt ctggtggagc tcctttggca aaagaagttg gcgaagcagt
     1201 cgctagaaga tttaatctac ccggtgtccg tcagggttac ggattaacag aaacgacatc
     1261 tgcatttatt attaccccag aaggtgatga taaacctgga gcatctggaa aagtagtacc
     1321 cttattcaaa gtaaaagtta ttgatcttga cactaaaaaa actttgggtg tcaaccgacg
     1381 aggagagatc tgtgtaaaag gacccagtct tatgttaggc tactcgaaca atccggaagc
     1441 aacaagagaa actattgatg aagagggttg gttgcacaca ggagatattg gatattacga
     1501 cgaagacgaa catttcttca ttgtcgaccg tttgaaatca ttaatcaaat acaaggggta
     1561 ccaggtacca cctgctgaat tggaatccgt tcttttgcaa catccaaata tatttgatgc
     1621 tggtgtggct ggtgtccccg atcccgatgc tggcgaactt ccaggggctg tagttgtaat
     1681 ggaaaaagga aaaactatga ctgaaaagga aattgtggat tatgttaata gtcaagtagt
     1741 gaaccacaaa cgtctgcgtg gtggcgttcg ttttgtggat gaagtaccta aaggtctaac
     1801 tggtaaaatt gatgctaaag taattagaga aattcttaag aaaccacaat cgggcccact
     1861 cgaggccgtg taataattct agagtcgggg cggccggccg cttcgagcag acatgataag
     1921 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1981 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2041 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2101 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga ccgatgccct
     2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     2281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3181 atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta aaccactgca
     3241 gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta tttcgttcgt
     3301 ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag
     3361 gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa
     3421 tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg tccgcctcca
     3481 tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc
     3541 gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc ggtatggctt
     3601 cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata ttatgaagaa
     3661 atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc gcggtgttgt
     3721 cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc gtaagatgct
     3781 tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa
     3841 gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt actttgaaag
     3901 tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg ccgctattga
     3961 gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct tttactttca
     4021 ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg
     4081 cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga agcatttatc
     4141 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
     4201 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     4261 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     4321 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
