pGFPm-T vector (V006032)

LOCUS       40924_21608        3419 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPm-T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Luo K, Harding SA, Tsai CJ.
  TITLE     A modified T-vector for simplified assembly of hairpin RNAi 
            constructs
  JOURNAL   Biotechnol. Lett. 30 (7), 1271-1274 (2008)
  PUBMED    18317701
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Luo K-M., Harding SA, Tsai C-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2007) School of Forest Resources and Environmental
            Science, Michigan Technological University, 1400 Townsend Drive, 
            Houghton, MI 49931-1295, USA
REFERENCE   3  (bases 1 to 3419)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2008"; volume: "30"; issue: "7"; pages: "1271-1274"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2007) School of Forest Resources and Environmental Science, 
            Michigan Technological University, 1400 Townsend Drive, Houghton, MI
            49931-1295, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        266..283
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    310..373
                     /note="multiple cloning site; includes SwaI, BamHI, XcmI,
                     XcmI, SpeI and AscI"
     promoter        complement(411..429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             450..1163
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     promoter        1514..1618
                     /label=AmpR promoter
     CDS             1619..2476
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2650..3238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"