LOCUS       40924_21608        3419 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGFPm-T, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Luo K, Harding SA, Tsai CJ.
  TITLE     A modified T-vector for simplified assembly of hairpin RNAi 
            constructs
  JOURNAL   Biotechnol. Lett. 30 (7), 1271-1274 (2008)
  PUBMED    18317701
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Luo K-M., Harding SA, Tsai C-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2007) School of Forest Resources and Environmental
            Science, Michigan Technological University, 1400 Townsend Drive, 
            Houghton, MI 49931-1295, USA
REFERENCE   3  (bases 1 to 3419)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2008"; volume: "30"; issue: "7"; pages: "1271-1274"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2007) School of Forest Resources and Environmental Science, 
            Michigan Technological University, 1400 Townsend Drive, Houghton, MI
            49931-1295, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        266..283
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    310..373
                     /note="multiple cloning site; includes SwaI, BamHI, XcmI,
                     XcmI, SpeI and AscI"
     promoter        complement(411..429)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             450..1163
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     promoter        1514..1618
                     /label=AmpR promoter
     CDS             1619..2476
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2650..3238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agacgattta ggtgacacta taggatatcg cggccgccta
      301 gaccatggat caatttaaat ggatccccat ggttttgttg gccatgatat ccatggacta
      361 gtggcgcgcc ataccaacaa aaccatggcc gggtacgcgg ccgcgatatc cctatagtga
      421 gtcgtattac agatctggta ccggtaaaaa tgagtaaagg agaagaactt ttcactggag
      481 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      541 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      601 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
      661 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
      721 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
      781 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
      841 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
      901 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgccacaaca
      961 ttgaagatgg ttcggttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1021 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
     1081 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     1141 atggcatgga tgagctctac aaataatgaa ttctttcgtc tcgcgcgttt cggtgatgac
     1201 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     1261 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     1321 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     1381 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg
     1441 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1501 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1561 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1621 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1681 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1741 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1801 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1861 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1921 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1981 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2041 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2101 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2161 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2221 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2281 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2341 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2401 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2461 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2521 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2581 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2641 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2701 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2761 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2821 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2881 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2941 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3001 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3061 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3121 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3181 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3241 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3301 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3361 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
