pGEX-MjpAzFRS_TetR vector (V006050)

LOCUS       V006050                 5628 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006050
VERSION     V006050
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Zhang Y, Ptacin JL, Fisher EC, Caffaro CE, San Jose K, Feldman AW,
            Turner CR, Romesberg FE.
  TITLE     A semi-synthetic organism that stores and retrieves increased
            genetic information
  JOURNAL   Nature (2017) In press
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Zhang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) Department of Chemistry, The Scripps
            Research Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA
            92037, USA
REFERENCE   3  (bases 1 to 5628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-SEP-2017) Department of Chemistry, The Scripps Research
            Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 92037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..109
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    117..133
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             162..1079
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     promoter        1389..1493
                     /label="AmpR promoter"
     CDS             1494..2351
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2525..3113
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3352..3972
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      complement(3997..4083)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        4399..4476
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4477..5556
                     /label="lacI"
                     /note="lac repressor"