LOCUS       V006050                 5628 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006050
VERSION     V006050
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Zhang Y, Ptacin JL, Fisher EC, Caffaro CE, San Jose K, Feldman AW,
            Turner CR, Romesberg FE.
  TITLE     A semi-synthetic organism that stores and retrieves increased
            genetic information
  JOURNAL   Nature (2017) In press
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Zhang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2017) Department of Chemistry, The Scripps
            Research Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA
            92037, USA
REFERENCE   3  (bases 1 to 5628)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5628)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature
            (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-SEP-2017) Department of Chemistry, The Scripps Research
            Institute, 10550 N Torrey Pines Rd, BCC526, San Diego, CA 92037,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..109
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    117..133
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             162..1079
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     promoter        1389..1493
                     /label="AmpR promoter"
     CDS             1494..2351
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2525..3113
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3352..3972
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     terminator      complement(3997..4083)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        4399..4476
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4477..5556
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
       61 atattaggca ccccaggctt tacactttat gcttccggct ggtataatgt gtggaattgt
      121 gagcggataa caatttcaca caggaaacag tattcagatc tatggacgag ttcgaaatga
      181 ttaaacgcaa caccagcgaa attatctctg aagaagagct gcgcgaggtg ctgaagaaag
      241 acgagaagag cgcgactatt ggctttgagc cgtccggtaa aattcacctg ggtcactacc
      301 tgcaaatcaa gaagatgatt gatctgcaaa acgctggttt tgacatcatt atcctgctgg
      361 cggacctgca cgcctacctg aatcaaaagg gcgagctgga tgagattcgc aagatcggcg
      421 actacaataa gaaagtcttc gaagccatgg gtttgaaggc taaatacgtc tacggtagca
      481 attttcagct ggataaggat tacacgttga atgtgtaccg tctggcgctg aaaaccacgc
      541 tgaaacgcgc ccgtcgttcc atggagctga ttgcgcgcga ggatgagaat ccaaaagttg
      601 ctgaggttat ttaccctatt atgcaagtta atccgttgca ctaccagggt gttgatgttg
      661 ccgtcggtgg tatggagcaa cgcaaaattc acatgctggc acgtgaactg ctgccgaaaa
      721 aggttgtctg tattcataat ccggtcctga ccggcctgga tggcgagggt aaaatgagca
      781 gcagcaaggg taactttatt gcagttgacg atagcccgga agaaatccgt gcgaagatca
      841 agaaagcgta ctgcccggca ggcgtggttg agggtaaccc gatcatggaa atcgccaagt
      901 attttctgga atacccactg acgattaagc gcccggagaa atttggcggc gacctgaccg
      961 tcaacagcta cgaggagctg gaaagcttgt ttaagaacaa agaactgcat ccgatgcgcc
     1021 tgaaaaacgc cgtggcggaa gagctgatta agattctgga accaattcgc aaacgtctgt
     1081 aagtcgactg atgactgacg atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     1141 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     1201 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     1261 cacgtagcga tagcggagtg tataattctt gaagacgaaa gggcctcgtg atacgcctat
     1321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3181 ctgcgttatc cccgagctct tagcgcgaat tgtcgaggga aattttttct aaatacattc
     3241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attaaatatg
     3301 gctggttctc gcagaaagaa acatatccat gaaatcccgc cccgaattga tatgtccaga
     3361 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
     3421 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
     3481 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
     3541 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
     3601 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
     3661 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
     3721 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
     3781 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
     3841 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
     3901 ccagccttct tattcggcct tgaattgatc atttgcggat tagaaaaaca acttaaatgt
     3961 gaaagtgggt cttaagcact aggtctaggg cggcggattt gtcctactca ggagagcgtt
     4021 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     4081 ttgatgcctc tagcacgcgt agagctagag ccttcaaccc agtcagctcc ttccggtggg
     4141 cgcggggcat gactaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc
     4201 tacatttgaa gagataaatt gcactgaaat ctagatgatt ctgtggataa ccgtattacc
     4261 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4321 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4381 tcacaccgca taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga
     4441 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
     4501 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     4561 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     4621 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     4681 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     4741 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     4801 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     4861 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     4921 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     4981 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     5041 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     5101 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     5161 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     5221 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     5281 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     5341 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     5401 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     5461 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5521 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa tctgtaacag
     5581 agcattagcg caaggtgatt tttgtcttct tgcgctaatt ttttcgct
//
