pGemT7cat vector (V006059)

LOCUS       V006059                 6753 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006059
VERSION     V006059
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
   PUBMED   20453134
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho,
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 6753)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13";
            pages: "4553-4556"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box
            445031, Moscow, ID 83844, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(72..728)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(729..831)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(897..3545)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     promoter        complement(3624..3642)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3660..3676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3684..3700)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3708..3738)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3753..3774)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4062..4650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4824..5681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5682..5786)
                     /label="AmpR promoter"
     rep_origin      complement(6118..6573)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6714..6730
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6737..6753
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"