LOCUS       V006059                 6753 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006059
VERSION     V006059
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Dual reporter system for in situ detection of plasmid transfer under
            aerobic and anaerobic conditions
  JOURNAL   Appl. Environ. Microbiol. 76 (13), 4553-4556 (2010)
   PUBMED   20453134
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Krol JE, Rogers LM, Krone SM, Top EM.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2010) Biological Sciences, University of Idaho,
            P.O. Box 445031, Moscow, ID 83844, USA
REFERENCE   3  (bases 1 to 6753)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2010"; volume: "76"; issue: "13";
            pages: "4553-4556"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2010) Biological Sciences, University of Idaho, P.O. Box
            445031, Moscow, ID 83844, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(72..728)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(729..831)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             complement(897..3545)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     promoter        complement(3624..3642)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3660..3676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3684..3700)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3708..3738)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3753..3774)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4062..4650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4824..5681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5682..5786)
                     /label="AmpR promoter"
     rep_origin      complement(6118..6573)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6714..6730
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6737..6753
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcggta cccggatccc ccaggcgttt aagggcacca ataactgcct
       61 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      121 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      181 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      241 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
      301 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      361 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      421 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
      481 gccagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
      541 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
      601 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
      661 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
      721 tcctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
      781 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacatctcat
      841 tttcgccaaa agtggcccag ggcttcccgg tatcaacagg gacaccagga tccttacgcg
      901 aacgcgaagt ccgactctaa gatgtcacgg aggttcaagt tacctttagc cggaagtgct
      961 ggcattttgt ccaattgaga ctcgtgcaac tggtcagcga actggtcgta gaaatcagcc
     1021 agtacatcac aagactcata tgtgtcaacc atagtttcgc gcactgcttt gaacaggttc
     1081 gcagcgtcag ccggaatggt accgaaggag tcgtgaatca gtgcaaaaga ttcgattccg
     1141 tacttctcgt gtgcccacac tacagtctta cgaaggtggc taccgtcttg gctgtgtaca
     1201 aagttaggag cgataccaga ctcctgtttg tgtgcatcaa tctcgctatc tttgttggtg
     1261 ttaatggtag gctgtaagcg gaactgaccg aggaacatca ggttcaagcg cgtctgaata
     1321 ggcttcttgt attcctgcca cacagggaaa ccatcaggag ttacccaatg cacagcgcaa
     1381 cgcttgcgaa gaatctctcc agtcttctta tctttgacct cagcagccag cagcttagca
     1441 gcagacttaa gccagttcat tgcttcaacc gcagctacca ccgtcacgct cacagattcc
     1501 caaatcagct tagccatgta tccagcagcc tgattcggct gagtgaacat cagacccttg
     1561 ccggaatcaa tagctggctg aatggtatct tccagcactt gttgacggaa gccgaactct
     1621 ttggacccgt aagccagcgt catgactgaa cgcttagtca cactgcgagt aacaccgtaa
     1681 gccagccatt gaccagccag tgccttagtg cccagcttga ctttctcaga gatttcacca
     1741 gtgttctcat cggtcacggt aactacttcg ttatcggtcc cattgattgc gtctgcttgt
     1801 agaatctcgt tgactttctt agcaacaatc ccgtagatgt cctgaacggt ttcactagga
     1861 agcaagttaa ccgcgcgacc acctacctca tctcggagca tcgcggagaa gtgctggatg
     1921 ccagagcaag acccgtcaaa cgccagcgga agggagcagt tatagctcag gccgtggtgc
     1981 tgtaccccag cgtactcaaa gcagaacgca aggaagcaga acggagaatc ttgctcagcc
     2041 caccaagtgt tctccagtgg agacttagcg caagccatga tgttctcgtg gttttcctca
     2101 atgaacttga tgcgctcagg gaacggaacc ttatcgacac ccgcacagtt tgcaccgtgg
     2161 attttcagcc agtagtaacc ttccttaccg attggtttac ctttcgccag cgtaagcagt
     2221 cctttggtca tatcgttacc ttgcgggttg aacattgaca cagcgtaaac acgaccgcgc
     2281 cagtccatgt tgtaagggaa ccagatggcc ttatggttag caaacttatt ggcttgctca
     2341 agcatgaact caaggctgat acggcgagac ttgcgagcct tgtccttgcg gtacacagca
     2401 gcggcagcac gtttccacgc ggtgagagcc tcaggattca tgtcgatgtc ttccggtttc
     2461 atcgggagtt cttcacgctc aatcgcaggg atgtcctcga ccggacaatg cttccacttg
     2521 gtgattacgt tggcgaccgc taggactttc ttgttgattt tccatgcggt gttttgcgca
     2581 atgttaatcg ctttgtacac ctcaggcatg taaacgtctt cgtagcgcat cagtgctttc
     2641 ttactgtgag tacgcaccag cgccagagga cgacgaccgt tagcccaata gccaccacca
     2701 gtaatgccag tccacggctt aggaggaact acgcaaggtt ggaacatcgg agagatgcca
     2761 gccagcgcac ctgcacgggt tgcgatagcc tcagcgtatt caggtgcgag ttcgatagtc
     2821 tcagagtctt gacctactac gccagcattt tggcggtgta agctaaccat tccggttgac
     2881 tcaatgagca tctcgatgca gcgtactcct acatgaatag agtcttcctt atgccacgaa
     2941 gaccacgcct cgccaccgag tagaccctta gagagcatgt cagcctcgac aacttgcata
     3001 aatgctttct tgtagacgtg ccctacgcgc ttgttgagtt gttcctcaac gtttttcttg
     3061 aagtgcttag cttcaaggtc acggatacga ccgaagcgag cctcgtcctc aatggcccga
     3121 ccgattgcgc ttgctacagc ctgaacggtt gtattgtcag cactggttag gcaagccaga
     3181 gtggtcttaa tggtgatgta cgctacggct tccggcttga tttcttgcag gaactggaag
     3241 gctgtcgggc gcttgccgcg cttagctttc acttcctcaa accagtcgtt gatgcgtgca
     3301 atcatcttag ggagtagggt agtgatgaga ggcttggcgg cagcgttatc cgcaacctca
     3361 ccagctttaa gttgacgctc aaacatcttg cggaagcgtg cttcacccat ctcgtaagac
     3421 tcatgctcaa gggccaactg ttcgcgagct aaacgctcac cgtaatggtc agccagagtg
     3481 ttgaacggga tagcagccag ttcgatgtca gagaagtcgt tcttagcgat gttaatcgtg
     3541 ttcatttagt gcctcttcca gttagtaaat ccgggatcag atcccgaacg ccatatagtg
     3601 agtcgtatta aaagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
     3661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4501 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5821 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5881 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     5941 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6001 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     6061 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     6121 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     6181 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     6241 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     6301 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     6361 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     6421 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6481 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6541 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     6601 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     6661 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     6721 gacggccagt gaattgtaat acgactcact ata
//
