pGAP-Km vector (V006093)

LOCUS       40924_21080        7890 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Agrobacterium binary gap repair vector pGAP-Km, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Bitrian M, Roodbarkelari F, Horvath M, Koncz C.
  TITLE     BAC-recombineering for studying plant gene regulation: developmental
            control and cellular localization of SnRK1 kinase subunits
  JOURNAL   Plant J. 65 (5), 829-842 (2011)
  PUBMED    21235649
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Agrobacterium binary gap repair vectors for recombineering with 
            Arabidopsis BACs
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7890)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Plant Developmental Biology, Max-Planck 
            Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 
            Cologne, NRW 50829, Germany
REFERENCE   4  (bases 1 to 7890)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2011"; volume: "65"; issue: "5"; pages: "829-842"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2008) Plant Developmental Biology, Max-Planck Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, NRW 50829, 
            Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /note="polylinker: EcoRI, HindIII, HindIII, XbaI, BglII,
                     PstI, SalI, BamHI, BstEII"
     misc_feature    complement(58..617)
                     /note="Agrobacterium str. Ach5 Ti plasmid T-DNA encoded
                     octopine synthase gene polyadenylation sequences"
     CDS             complement(793..1581)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1629..1808)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1932..1956
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            complement(3159..3268)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4062..4773
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4778..5073
                     /note="derived from Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621)"
     misc_feature    5360..5384
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      complement(5872..6460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6634..7491)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7492..7596)
                     /label=AmpR promoter