LOCUS       40924_21080        7890 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Agrobacterium binary gap repair vector pGAP-Km, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Bitrian M, Roodbarkelari F, Horvath M, Koncz C.
  TITLE     BAC-recombineering for studying plant gene regulation: developmental
            control and cellular localization of SnRK1 kinase subunits
  JOURNAL   Plant J. 65 (5), 829-842 (2011)
  PUBMED    21235649
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Agrobacterium binary gap repair vectors for recombineering with 
            Arabidopsis BACs
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7890)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Plant Developmental Biology, Max-Planck 
            Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 
            Cologne, NRW 50829, Germany
REFERENCE   4  (bases 1 to 7890)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7890)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2011"; volume: "65"; issue: "5"; pages: "829-842"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2008) Plant Developmental Biology, Max-Planck Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, NRW 50829, 
            Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /note="polylinker: EcoRI, HindIII, HindIII, XbaI, BglII,
                     PstI, SalI, BamHI, BstEII"
     misc_feature    complement(58..617)
                     /note="Agrobacterium str. Ach5 Ti plasmid T-DNA encoded
                     octopine synthase gene polyadenylation sequences"
     CDS             complement(793..1581)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1629..1808)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    1932..1956
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            complement(3159..3268)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4062..4773
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4778..5073
                     /note="derived from Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621)"
     misc_feature    5360..5384
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      complement(5872..6460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6634..7491)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7492..7596)
                     /label=AmpR promoter
ORIGIN
        1 gaattccaag cttggccaag cttctagaga tctgcaggtc gacggatccg ggttacccgg
       61 ccgccgtgct ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata
      121 ctcaacttca aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga
      181 gcgttattag ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat
      241 ttgaataaga tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa
      301 ttggatttgt aataataaaa cgcaattgtt tgttattgtg gcgctctatc atagatgtcg
      361 ctataaacct attcagcaca atatattgtt ttcattttaa tattgtacat ataagtagta
      421 gggtacaatc agtaaattga acggagaata ttattcataa aaatacgata gtaacgggtg
      481 atatattcat tagaatgaac cgaaaccggc ggtaaggatc tgagctacac atgctcaggt
      541 tttttacaac gtgcacaaca gaattgaaag caaatatcat gcgatcatag gcgtctcgca
      601 tatctcatta aagcaggggg tgggcgaaga actccagcat gagatccccg cgctggagga
      661 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
      721 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
      781 gagtcccgct cagaagaact cgtcaagaaa gcgatagaag gcgatgcgct gcgaatcggg
      841 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
      901 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
      961 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     1021 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     1081 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     1141 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     1201 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     1261 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     1321 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     1381 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     1441 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     1501 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     1561 gtgcaatcca tcttgttcaa tccacatgat catgggccgg atctggattg agagtgaata
     1621 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
     1681 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     1741 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt
     1801 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     1861 acgtgactcc cttaattctc cgctcatgat cagattgtcg tttcccgcct tcggtttaaa
     1921 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     1981 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     2041 tgccaaccac agggttcccc tcgggagtgc ttggcattcc gtgcgataat gacttctgtt
     2101 caaccaccca aacgtcggaa agcctgacga cggagcagca ttccaaaaag atcccttggc
     2161 tcgtctgggt cggctagaag gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg
     2221 cggacgtagc gcagcgccga aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg
     2281 atctgcttgt cgctctttcg gccgacgtcc tggccagtca tcacgcgcca aagttccgtc
     2341 acaggatgat ctggcgcgag ttgctggatc tcgccttcaa tccgggtctg tggcgggaac
     2401 tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt gcctgggcag
     2461 tcgccgccga cgccgttgat gtggacgccg ggcccgatca tattgtcgct caggatcgtg
     2521 gcgttgtgct tgtcggccgt tgctgtcgta atgatatcgg caccttcgac cgcctgttcc
     2581 gcagaggtgc aggcctcgat ctgaaacccg aaccgctgga gattgcggga gcagcgagca
     2641 gtagcctcgg ggtcgatgtc gtaaagtcgt atccgatcga cgccgatcag cgccttgaag
     2701 gccaaagcct ggaactcact ttgggcaccg ttgccgatca gcgccatcgt gcgcgaatct
     2761 ttacgggcca gatactttgc cgcgatcgcg gaggtcgcgg ccgttcgcaa ggccgtcagg
     2821 attgtcattt ccgacagcag cagcggatag ccgctatcga catcggagag cacgccgaac
     2881 gcggttaccg gcctcttcat cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc
     2941 ctggcggacg ggaagtatcc agctcgagat ccggattttg tagccctggc cgacggccag
     3001 caggtaggcc gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc
     3061 gtctggaagg cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc
     3121 agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg
     3181 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     3241 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     3301 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     3361 aatcgctata atgaccccga agcagggtta tgcagcggaa aagcgctgct tccctgctgt
     3421 tttgtggaat atctaccgac tggaaacagg caaatgcagg aaattactga actgagggga
     3481 caggcgagag acgatgccaa agagctacac cgacgagctg gccgagtggg ttgaatcccg
     3541 cgcggccaag aagcgccggc gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga
     3601 tgtcgaggcg gcgttagcgt ccggctatgc gctcgtcacc atttgggagc acatgcggga
     3661 aacggggaag gtcaagttct cctacgagac gttccgctcg cacgccaggc ggcacatcaa
     3721 ggccaagccc gccgatgtgc ccgcaccgca ggccaaggct gcggaacccg cgccggcacc
     3781 caagacgccg gagccacggc ggccgaagca ggggggcaag gctgaaaagc cggcccccgc
     3841 tgcggccccg accggcttca ccttcaaccc aacaccggac aaaaaggatc tagcgtggac
     3901 tcaaggctct cgcgaatggc tcgcgttgga aactttcatt gacacttgag gggcaccgca
     3961 gggaaattct cgtccttgcg agaaccggct atgtcgtgct gcgcatcgag cctgcgccct
     4021 tggcttgtct cgcccctctc cgcgtcgcta cggggcttcc agcgcctttc cgacgctcac
     4081 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
     4141 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga
     4201 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
     4261 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
     4321 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
     4381 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
     4441 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
     4501 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
     4561 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
     4621 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
     4681 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
     4741 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc agtccttgcc attgccggga
     4801 tcggggcagt aacgggatgg gcgatcagcc cgagcgcgac gcccggaagc attgacgtgc
     4861 cgcaggtgct ggcatcgaca ttcagcgacc aggtgccggg cagtgagggc ggcggcctgg
     4921 gtggcggcct gcccttcact tcggccgtcg gggcattcac ggacttcatg gcggggccgg
     4981 caatttttac cttgggcatt cttggcatag tggtcgcggg tgccgtgctc gtgttcgggg
     5041 gtgaattgca agctagcttg cttggtcgtt ccggtaccgt gaacgtcggc tcgattgtac
     5101 ctgcgttcaa atactttgcg atcgtgttgc gcgcctgccc ggtgcgtcgg ctgatctcac
     5161 ggatcgactg cttctctcgc aacgccatcc gacggatgat gtttaaaagt cccatgtgga
     5221 tcactccgtt gccccgtcgc tcaccgtgtt ggggggaagg tgcacatggc tcagttctca
     5281 atggaaatta tctgcctaac cggctcagtt ctgcgtagaa accaacatgc aagctccacc
     5341 gggtgcaaag cggcagcggc ggcaggatat attcaattgt aaatggcttc atgtccggga
     5401 aatctacatg gatcagcaat gagtatgatg gtcaatatgg agaaaaagaa agagtaatta
     5461 ccaatttttt ttcaattcaa aaatgtagat gtccgcagcg ttattataaa atgaaagtac
     5521 attttgataa aacgacaaat tacgatccgt cgtatttata ggcgaaagca ataaacaaat
     5581 tattctaatt cggaaatctt tatttcgacg tgtctacatt cacgtccaaa tgggggctta
     5641 gatgagaaac ttcacgatcg atgggagagg cggtttgcgt attgggcgct cttccgcttc
     5701 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5761 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5821 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5881 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5941 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6001 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6061 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6121 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6181 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6241 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6301 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6361 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6421 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6481 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6541 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6601 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6661 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6721 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6781 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6841 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6901 aagtagttcg ccagttaata gtttgcgcaa cgttgtttgc attgctacag gcatcgtggt
     6961 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7021 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7081 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7141 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7201 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7261 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7321 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7381 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7441 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7501 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7561 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7621 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7681 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7741 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7801 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7861 gccatcgccc tgatagacgg tttttcgccc
//
