pGAP-Hyg vector (V006094)

LOCUS       40924_21075        7942 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Agrobacterium binary gap repair vector pGAP-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Bitrian M, Roodbarkelari F, Horvath M, Koncz C.
  TITLE     BAC-recombineering for studying plant gene regulation: developmental
            control and cellular localization of SnRK1 kinase subunits
  JOURNAL   Plant J. 65 (5), 829-842 (2011)
  PUBMED    21235649
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Agrobacterium binary gap repair vectors for recombineering with 
            Arabidopsis BACs
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7942)
  AUTHORS   Bitrian M, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Plant Developmental Biology, Max-Planck 
            Institute for Plant Breeding Research, Carl-von-Linne-Weg 10, 
            Cologne, NRW 50829, Germany
REFERENCE   4  (bases 1 to 7942)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2011"; volume: "65"; issue: "5"; pages: "829-842"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2008) Plant Developmental Biology, Max-Planck Institute for 
            Plant Breeding Research, Carl-von-Linne-Weg 10, Cologne, NRW 50829, 
            Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..59
                     /note="polylinker: EcoRI, HindIII, HindIII, XbaI, BglII,
                     PstI, SalI, XbaI, BamHI"
     misc_feature    64..383
                     /note="derived from Escherichia coli transposon Tn5
                     sequence in GenBank Accession Number U00004"
     promoter        467..646
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             691..1713
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      1747..1999
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2059..2083
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     oriT            complement(3211..3320)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      4114..4825
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4829..5124
                     /note="derived from Birmingham IncP-alpha plasmid RK2 
                     (gb|L27758.1|BIACOMGEN; NC_001621)"
     misc_feature    5412..5436
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6686..7543)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7544..7648)
                     /label=AmpR promoter